Formation of a heterooctameric complex between aspartate α-decarboxylase and its cognate activating factor, PanZ, is CoA-dependent.
Formation of a heterooctameric complex between aspartate α-decarboxylase and its cognate activating factor, PanZ, is CoA-dependent.
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天冬氨酸α-二羧化酶及其同源激活因子Panz之间形成异摄氏度复合物是COA依赖性的。
DOI：
10.1016/j.bbrc.2012.08.084
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发表时间：
2012-09-28
影响因子：
3.1
通讯作者：
Webb, Michael E.
中科院分区：
文献类型：
--
作者：
Monteiro, Diana C. F.;Rugen, Michael D.;Shepherd, Dale;Nozaki, Shingo;Niki, Hironori;Webb, Michael E.
► PanZ is required for formation of the pyruvoyl-cofactor in PanD (ADC) in vivo. ► PanZ and PanD interact with nanomolar affinity. ► Interaction of PanZ and PanD is dependent upon coenzyme A. ► PanZ and PanD form a heterooctameric complex which binds four molecules of CoA. The existence of a fifth essential protein for pantothenate biosynthesis in some enteric bacteria has recently been reported by Stuecker et al. and Nozaki et al. (in press). This protein, PanZ, catalyses the activation of the PanD zymogen to form ADC and is essential for prototrophic growth. In this paper, we characterise the interaction of PanZ with coenzyme A and a constitutively inactive mutant of PanD using a combination of isothermal titration calorimetry and mass spectrometry. These approaches reveal that the two proteins interact with nanomolar affinity in a CoA-dependent fashion to form a heterooctameric complex.
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影响因子：
11.4
作者：
Schmitzberger, F;Kilkenny, ML;Blundell, TL
通讯作者：
Blundell, TL
影响因子：
1.6
作者：
Studier, FW
通讯作者：
Studier, FW
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作者：
Trip, Hein;Mulder, Niels L.;Lolkema, Juke S.
通讯作者：
Lolkema, Juke S.
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作者：
Saldanha, SA;Birch, LM;Abell, C
通讯作者：
Abell, C
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6.4
作者：
Stuecker, Tara N.;Tucker, Alex C.;Escalante-Semerena, Jorge C.
通讯作者：
Escalante-Semerena, Jorge C.