Polysorbate cationic synthetic vesicle for gene delivery.
Polysorbate cationic synthetic vesicle for gene delivery.
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DOI：
10.1002/jbm.a.32999
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发表时间：
2011-03-01
影响因子：
4.9
通讯作者：
Liang, Wenquan
中科院分区：
文献类型：
--
作者：
Huang, Yongzhuo;Rao, Yuefeng;Chen, Jinliang;Yang, Victor C.;Liang, Wenquan
合成非离子表面活性剂囊泡(微球)是一种具有封闭双层结构的胶体体系，与脂质体相比，在稳定性和成本方面显示出明显的优势。本文将聚山梨酸阳离子微球(PCN)作为基因载体。PCN由非离子表面活性剂(即聚山梨酸酯)和阳离子胆固醇组成，采用薄膜水合方法合成。所制备的微球形态规则，颗粒尺寸为100~200 nm，Zeta电位为+30~45 mV。PCN在室温下4周内表现出良好的物理稳定性。用凝胶阻滞法测定了PCN与寡脱氧核苷酸的结合能力，结果表明，当+/−电荷比大于或等于4时，PCN形成离子络合物。基因转移研究表明，PCN在介导细胞摄取和转移DNA表达方面表现出很高的效率。基于这些发现，PCN可能提供作为一种有效的基因递送系统的潜力。
Synthetic nonionic surfactant vesicles (niosomes) are a colloidal system with closed bilayer structures, displaying distinct advantages in stability and cost compared to liposomes. In this paper, polysorbate cationic niosomes (PCNs) were developed as gene carriers. The PCNs comprised nonionic surfactants (i.e. polysorbates) and a cationic cholesterol, and were synthesized using a film hydration method. The niosomes thus prepared possessed a regular morphology, and a particle size of 100 ~ 200 nm, and a zeta potential of +30 ~ 45 mV. The PCNs showed great physical stability over the course of 4 weeks at room temperature. The binding capacity of PCNs toward oligodeoxynucleotides (ODN) was assessed by a gel retardation approach, which demonstrated that the ionic complexes were formed when +/− charge ratio reached to 4 or greater. Gene transfer study showed that the PCNs exhibited a high efficiency in mediating cellular uptake and transferred DNA expression. Based on these findings, PCNs may offer the potential to function as an effective gene delivery system.
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影响因子：
3.7
作者：
Liu, F;Yang, JP;Liu, DX
通讯作者：
Liu, DX
影响因子：
9.9
作者：
Devaraj, GN;Parakh, SR;Rambhau, D
通讯作者：
Rambhau, D
DOI：
10.1007/s10856-007-3193-4
发表时间：
2008-02-01
影响因子：
3.7
作者：
Huang, Yongzhuo;Chen, Jinliang;Liang, Wenquan
通讯作者：
Liang, Wenquan
影响因子：
3.8
作者：
Bayindir, Zerrin Sezgin;Yuksel, Nilufer
通讯作者：
Yuksel, Nilufer
影响因子：
3.3
作者：
Vangala, Anil;Kirby, Daniel;Perrie, Yvonne
通讯作者：
Perrie, Yvonne