Spectrum effect correlation of yangyin tongnao granules on cerebral ischemia-reperfusion injury rats.
Spectrum effect correlation of yangyin tongnao granules on cerebral ischemia-reperfusion injury rats.
复制标题
养阴通脑颗粒对脑缺血再灌注损伤大鼠的频谱效应相关性研究
DOI：
10.3389/fphar.2022.947978
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发表时间：
2022
影响因子：
5.6
通讯作者：
Wan, Haitong
中科院分区：
文献类型：
--
作者：
Zhang, Yangyang;Yu, Li;Yang, Jiehong;Ding, Zhishan;He, Yu;Wan, Haitong
关键词：
养阴通脑颗粒是一种中药复方制剂，对脑缺血再灌注损伤有较好的治疗作用。本研究旨在探讨脑缺血再灌注损伤大鼠血浆中益愈通颗粒活性成分与炎症反应的关系。采用高效液相色谱法（HPLC）测定不同时间点给药后大脑中动脉闭塞（MCAO）大鼠脑组织的指纹图谱。采用酶联免疫吸附法（ELISA）检测不同时间点MCAO大鼠血浆中白细胞介素-18（IL-18）和肿瘤坏死因子-α（TNF-α）的水平。采用灰色关联、多元线性回归和偏最小二乘法3种不同的数学模型，建立了YYTNG指纹图谱与体内炎症指标之间的光谱效应关系。结果表明，血浆中各色谱峰与炎症指数呈一定的相关性，其顺序为：P2 >P6 >P5 >P1 >P3 >P4。因此，本研究成功建立了益愈通颗粒对大鼠脑缺血再灌注损伤的谱效关系。研究结果为中药指纹图谱与药效学关系的分析提供了一定的指导思想。
Yangyin Tongnao Granules (YYTNG), as traditional Chinese medicine (TCM) compound preparation, have a good curative effect on cerebral ischemia-reperfusion injury. This study aimed to investigate the relationship between the active components of YYTNG in the plasma and the inflammatory response in cerebral ischemia-reperfusion injury rats. High-performance liquid chromatography (HPLC) was conducted to determine the fingerprints at different time points of middle cerebral artery occlusion (MCAO) rats after the administration of YYTNG at different times points. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the levels of interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) in the plasma of MCAO rats at different time points. The spectral-effect relationship between the YYTNG fingerprints and inflammatory indexes in vivo was established by combining three different mathematical models, grey correlation, multiple linear regression, and partial least-square method. The results revealed that each chromatographic peak in the HPLC of the plasma exhibited a certain correlation with the inflammatory index, in the following order: P2 >P6 >P5 >P1 >P3 >P4. Therefore, this study successfully established the spectrum-effect correlation of YYTNG on cerebral ischemia-reperfusion injury rats. The results provide a certain guiding ideology for the analyses of the relationship between fingerprints and the pharmacodynamics of TCM prescriptions.
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