Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer.
Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer.
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DOI：
10.1039/c0an00204f
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Patel V
中科院分区：
文献类型：
--
作者：
Rusling JF;Kumar CV;Gutkind JS;Patel V
这篇重要的综述评估了用于癌症检测和诊断的可行的护理点蛋白质生物标志物测量的进展。在医生的手术和诊所中测量特定的、选择性的癌症生物标志物蛋白质组的能力有可能彻底改变癌症检测、监测和治疗。这个梦想设想了可靠、廉价、自动化、技术要求不高的设备，可以在临床环境中分析病人的血清或唾液，从而进行现场诊断。用于蛋白质测定的现有商业产品在实验室环境中是可靠的，但对于即时应用具有限制。许多超灵敏的免疫传感器和一些阵列已经开发出来，其中许多是基于纳米技术。多标记检测结合免疫传感器和阵列中的高捕获分子密度似乎能够检测宽范围的蛋白质浓度，灵敏度范围为亚pg/mL−1水平。多标记阵列可以被设计为检测同一样品中的高丰度和超低丰度蛋白质。然而，只有少数较新的超灵敏度方法已经用真实的患者样本进行了评估，这是建立临床灵敏度和选择性的关键。
This critical review evaluates progress toward viable point-of-care protein biomarker measurements for cancer detection and diagnostics. The ability to measure panels of specific, selective cancer biomarker proteins in physicians’ surgeries and clinics has the potential to revolutionize cancer detection, monitoring, and therapy. The dream envisions reliable, cheap, automated, technically undemanding devices that can analyze a patient’s serum or saliva in a clinical setting, allowing on-the-spot diagnosis. Existing commercial products for protein assays are reliable in laboratory settings, but have limitations for point-of-care applications. A number of ultrasensitive immunosensors and some arrays have been developed, many based on nanotechnology. Multilabel detection coupled with high capture molecule density in immunosensors and arrays seems to be capable of detecting a wide range of protein concentrations with sensitivity ranging into the sub pg mL−1 level. Multilabel arrays can be designed to detect both high and ultralow abundance proteins in the same sample. However, only a few of the newer ultrasensitive methods have been evaluated with real patient samples, which is key to establishing clinical sensitivity and selectivity.
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影响因子：
3.4
作者：
Bock, C;Coleman, M;Zichi, D
通讯作者：
Zichi, D
DOI：
10.1196/annals.1384.005
发表时间：
2007-01-01
期刊：
ORAL-BASED DIAGNOSTICS
影响因子：
--
作者：
Gau, Vincent;Wong, David
通讯作者：
Wong, David
影响因子：
12.6
作者：
Dill, K;Montgomery, DD;Oleinikov, AV
通讯作者：
Oleinikov, AV
影响因子：
12.6
作者：
Bart, M;Stigter, ECA;van Bennekom, WP
通讯作者：
van Bennekom, WP
影响因子：
--
作者：
Bensmail H;Haoudi A
通讯作者：
Haoudi A