Antibiotic Resistance in Non-Typhoidal Salmonella enterica Strains Isolated from Chicken Meat in Indonesia.
Antibiotic Resistance in Non-Typhoidal Salmonella enterica Strains Isolated from Chicken Meat in Indonesia.
复制标题
从印度尼西亚鸡肉中分离的非伤寒沙门氏菌肠道菌株的抗生素耐药性。
DOI：
10.3390/pathogens11050543
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发表时间：
2022-05-04
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影响因子：
--
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中科院分区：
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这项研究证实了印度尼西亚非伤寒沙门氏菌（NTS）抗生素耐药性的增加。我们确认了从印度尼西亚鸡肉中分离的临床NTS（n = 50）的毒力基因和抗菌药物敏感性，并检测了抗菌药物耐药基因。在50株菌株中，30株（60%）对萘啶酸（NA）不敏感，且所有菌株均存在gyrA氨基酸突变。在27株四环素（TC）非敏感菌株中，22株（81.5%）具有tetA和/或tetB。对氨苄西林、庆大霉素、卡那霉素的不敏感率低于NA、TC，但blaTEM、aadA的检出率较高。与敏感菌株相比，非敏感菌株显示出高流行率的毒力基因（tcfA，p = 0.014; cdtB，p < 0.001; sfbA，p < 0.001; fimA，p = 0.002）。S. Schwarzengrund是最流行的血清型（23株，46%），也是最常检测到的多重耐药菌株。结果表明，S.除了上述基因外，Schwarzengrund在hlyE（p = 0.011）和phoP/Q（p = 0.011）方面显著高于其他血清型。总之，从印尼鸡中分离的NTS菌株对抗生素具有较高的耐药性，并具有多种毒力因子。特别是S. Schwarzengrund菌株最常被检测为多重抗菌药物耐药，并且具有较高的毒力基因流行率。
The increase in antibiotic resistance in non-typhoidal Salmonella enterica (NTS) has been confirmed in Indonesia by this study. We confirmed the virulence genes and antimicrobial susceptibilities of clinical NTS (n = 50) isolated from chicken meat in Indonesia and also detected antimicrobial resistance genes. Of 50 strains, 30 (60%) were non-susceptible to nalidixic acid (NA) and all of them had amino acid mutations in gyrA. Among 27 tetracycline (TC) non-susceptible strains, 22 (81.5%) had tetA and/or tetB. The non-susceptibility rates to ampicillin, gentamicin or kanamycin were lower than that of NA or TC, but the prevalence of blaTEM or aadA was high. Non-susceptible strains showed a high prevalence of virulence genes compared with the susceptible strains (tcfA, p = 0.014; cdtB, p < 0.001; sfbA, p < 0.001; fimA, p = 0.002). S. Schwarzengrund was the most prevalent serotype (23 strains, 46%) and the most frequently detected as multi-antimicrobial resistant. The prevalence of virulence genes in S. Schwarzengrund was significantly higher than other serotypes in hlyE (p = 0.011) and phoP/Q (p = 0.011) in addition to the genes above. In conclusion, NTS strains isolated from Indonesian chicken had a high resistance to antibiotics and many virulence factors. In particular, S. Schwarzengrund strains were most frequently detected as multi-antimicrobial resistant and had a high prevalence of virulence genes.
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