Non-thermal atmospheric pressure plasma increased mRNA expression of growth factors in human gingival fibroblasts
Non-thermal atmospheric pressure plasma increased mRNA expression of growth factors in human gingival fibroblasts
复制标题
非热常压等离子体增加人牙龈成纤维细胞生长因子mRNA表达
作者：
J. Kwon;Yong Hee Kim;E. Choi;Chong;Kyoung;K. Kim
本实验旨在探讨非热大气压等离子体射流（NTAPPJ）对人牙龈成纤维细胞（HGF）细胞活性的影响，为其在牙龈伤口愈合过程中可能的非手术应用提供依据。将HGF细胞与NTAPPJ接触1、2和4分钟，观察细胞附着、细胞活力、附着细胞形态、增殖率和各种生长因子的mRNA表达情况。此外，研究人员还利用NTAPPJ产生的化学物质的清除剂来识别造成这种影响的化学物质。暴露于NTAPPJ后，HGF细胞的附着数量和增殖没有明显变化。此外，所有HGF细胞暴露于NTAPPJ 1,2,4分钟后，细胞活力都很高。然而，NTAPPJ暴露后，细胞被拉伸更大，转化生长因子和血管内皮生长因子的mRNA表达量显著增加。此外，清道夫试验表明，一氧化氮很可能是导致细胞活性增加的化学物质。结果表明，NTAPPJ可增加人牙龈成纤维细胞中生长因子mRNA的表达。应用NTAPPJ将有助于临床牙龈伤口愈合，但还需要进一步的研究来证实其效果。
The aim of this in vitro study was to investigate the effects of a non-thermal atmospheric pressure plasma jet (NTAPPJ) on the cellular activity of human gingival fibroblasts (HGF) for possible non-surgical application of it during gingival wound healing. HGF cells were exposed with NTAPPJ for 1, 2, and 4 min and were investigated for cellular attachment, cell viability, morphology of attached cells, proliferation rate, and messenger ribonucleic acid (mRNA) expression of various growth factors. Also, scavengers for chemicals produced by NTAPPJ were used to identify the chemical species responsible for the effects. There was no significant change in the number of HGF cells attached or their proliferation following NTAPPJ exposure. Also, high cell viability resulted from exposure of all of HGF cells to NTAPPJ for 1, 2, and 4 min. However, cells were more stretched while the mRNA expressions of transforming growth factor and vascular endothelial growth factor were significantly increased following NTAPPJ exposure. Additionally, the scavenger test showed that nitric oxide is likely to be the chemical responsible for an increase of cellular activity. The results demonstrated that the NTAPPJ increased mRNA expressions of growth factors in human gingival fibroblasts. Application of NTAPPJ would be useful in gingival wound healing in clinics though additional studies confirming the effects would be needed.
DOI：
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发表时间：
2001
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
B. Geiger;A. Bershadsky;R. Pankov;K. Yamada
通讯作者：
B. Geiger;A. Bershadsky;R. Pankov;K. Yamada
DOI：
10.1073/pnas.0502977102
发表时间：
2005-09-13
影响因子：
11.1
作者：
Isenberg, JS;Ridnour, LA;Roberts, DD
通讯作者：
Roberts, DD