Inhibition of the expression of oncogene SRSF3 by blocking an exonic splicing suppressor with antisense oligonucleotides.
Inhibition of the expression of oncogene SRSF3 by blocking an exonic splicing suppressor with antisense oligonucleotides.
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通过反义寡核苷酸阻断外显子剪接抑制子来抑制癌基因 SRSF3 的表达
DOI：
10.1039/c7ra11267j
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发表时间：
2018-02-09
期刊：
影响因子：
3.9
通讯作者：
Jia, Rong
中科院分区：
文献类型：
--
作者：
Guo, Jihua;Che, Xiaoxuan;Wang, Xiaole;Jia, Rong
Antisense oligonucleotides (ASOs) have been widely used to regulate alternative splicing of pre-mRNA by targeting splice sites, branch points, or exonic splice enhancers to increase exon skipping or intron retention. So far, few studies have used ASOs to block exonic splicing suppressor (ESS) and increase exon inclusion. Previously, we demonstrated that serine and arginine rich splicing factor 3 (SRSF3) (also called SRp20) is an oncogene. The inclusion of its alternative exon 4 down-regulates its expression. An ESS motif is responsible for the skipping of alternative exon 4. Here, we used an economical method to screen effective anti-ESS ASO. We discovered that an ASO targeting the ESS motif can promote the inclusion of exon 4, reduce SRSF3 expression, and inhibit cell growth in oral cancer cells. Our results suggested that using anti-ESS ASOs can efficiently increase exon inclusion and be used as a potential anti-cancer drug. Inhibition of the expression of oncogene SRSF3 and cancer cell growth by blocking an exonic splicing suppressor in alternative exon 4 of SRSF3 with SR-3 antisense oligonucleotide.
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影响因子：
37.3
作者：
He X;Zhang P
通讯作者：
Zhang P
DOI：
10.1016/j.bbagrm.2013.11.006
发表时间：
2014-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Jang HN;Lee M;Loh TJ;Choi SW;Oh HK;Moon H;Cho S;Hong SE;Kim DH;Sheng Z;Green MR;Park D;Zheng X;Shen H
通讯作者：
Shen H
影响因子：
11.4
作者：
Sureau, A;Gattoni, R;Soret, J
通讯作者：
Soret, J
影响因子：
9.2
作者：
Shchelkunova A;Ermolinsky B;Boyle M;Mendez I;Lehker M;Martirosyan KS;Kazansky AV
通讯作者：
Kazansky AV
SRSF3的表达与口腔鳞状细胞癌的发生、进展相关
影响因子：
3.6
作者：
Peiqi L;Zhaozhong G;Yaotian Y;Jun J;Jihua G;Rong J
通讯作者：
Rong J