Scaffold-Free Biofabrication

Scaffold-Free Biofabrication
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无支架生物制造

DOI:
10.1007/978-3-319-40498-1_16-1
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发表时间:
2017
期刊:
3D Printing and Biofabrication
影响因子:
--
通讯作者:
Nakayama Koichi
Nakayama Koichi
中科院分区:
--
文献类型:
--
作者:
Verissimo Ana Raquel;Nakayama Koichi

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组织工程和再生医学在过去的十年中取得了巨大的科学,医学和技术进步。大多数方法结合联合收割机支架,如聚合物,和活细胞,使可植入的结构,将整合和愈合宿主的组织。最近,替代的无支架方法开始出现。本章概述了目前的无支架系统,优点,挑战,方法和应用。在实验室中使用患者自身细胞人工制造的无支架组织已经成功地用于小规模的心脏和血管再生。新的技术和方法正在开发中,不仅在组装细胞和结构方面,而且在新设备方面,即3D生物打印。原代和干细胞或iPSC衍生的细胞都用于组装目前正在体内和体外测试的人工组织。这些工程构建物具有许多应用,例如再生医学、疾病模型和药物测试。
Tissue engineering and regenerative medicine have met great scientific, medical, and technological advances in the past decade. Most methods combine scaffolds, such as polymers, and living cells to make implantable structures that will integrate and heal the host’s tissues. More recently, alternative scaffold-free approaches have started to emerge. This chapter provides an overview of the current scaffold-free systems, advantages, challenges, methods, and applications. Scaffold-free tissue artificially produced in the lab using patients’ own cells has already been successfully used in heart and blood vessel regeneration at a small scale. New techniques and approaches are being developed, not only in terms of assembling cells and structures but also in terms of new equipment, namely for 3D bioprinting. Both primary and stem or iPSC-derived cells are used to assemble artificial tissues that are currently being tested in vivo and in vitro. These engineered constructs have numerous applications, such as regenerative medicine, disease models, and drug testing.
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