Scaffold-free vascular tissue engineering using bioprinting.
Scaffold-free vascular tissue engineering using bioprinting.
复制标题
DOI：
10.1016/j.biomaterials.2009.06.034
复制
发表时间：
2009-10
期刊：
影响因子：
14
通讯作者：
Forgacs, Gabor
中科院分区：
文献类型：
--
作者：
Norotte, Cyrille;Marga, Francois S.;Niklason, Laura E.;Forgacs, Gabor
目前外源性支架或细胞外基质材料的局限性强调了对替代组织工程解决方案的需求。支架可能引起不良的宿主反应，干扰细胞间的直接相互作用，以及细胞产生的ECM的组装和排列。因此，生产无支架工程组织结构的制造技术最近出现了。在这里，我们报告了一种完全生物自组装的方法，我们通过快速原型生物打印方法实现了无支架的小直径血管重建。各种血管细胞类型，包括平滑肌细胞和成纤维细胞，聚集成离散的单位，要么是多细胞球体，要么是直径可控的圆柱体（300至500 μm）。这些是与琼脂糖棒一起一层一层地打印出来的，在这里用作成型模板。打印后，离散单元的融合产生单层和双层小直径血管管（外径从0.9到2.5 mm不等）。该方法的一个独特之处在于，它能够设计出不同形状的血管，以及将不同直径的管道组合在一起的分层树。该技术快速且易于扩展。
Current limitations of exogenous scaffolds or extracellular matrix based materials have underlined the need for alternative tissue-engineering solutions. Scaffolds may elicit adverse host responses and interfere with direct cell-cell interaction, as well as assembly and alignment of cell-produced ECM. Thus, fabrication techniques for production of scaffold-free engineered tissue constructs have recently emerged. Here we report on a fully biological self-assembly approach, which we implement through a rapid prototyping bioprinting method for scaffold-free small diameter vascular reconstruction. Various vascular cell types, including smooth muscle cells and fibroblasts, were aggregated into discrete units, either multicellular spheroids or cylinders of controllable diameter (300 to 500 μm). These were printed layer-by-layer concomitantly with agarose rods, used here as a molding template. The post-printing fusion of the discrete units resulted in single- and double-layered small diameter vascular tubes (OD ranging from 0.9 to 2.5 mm). A unique aspect of the method is the ability to engineer vessels of distinct shapes and hierarchical trees that combine tubes of distinct diameters. The technique is quick and easily scalable.
登录
查看更多内容
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
DOI：
10.1073/pnas.0400164101
发表时间：
2004-03-02
影响因子：
11.1
作者：
Jakab, K;Neagu, A;Forgacs, G
通讯作者：
Forgacs, G
影响因子：
4.7
作者：
Boland, Thomas;Xu, Tao;Cui, Xiaofeng
通讯作者：
Cui, Xiaofeng
影响因子：
2.1
作者：
Marga, Francoise;Neagu, Adrian;Forgacs, Gabor
通讯作者：
Forgacs, Gabor
影响因子：
4.9
作者：
Higgins, SP;Solan, AK;Niklason, LE
通讯作者：
Niklason, LE