Isolation of the canine inhibin βB subunit gene and characterization of signalling mediated by canine inhibin βB
Isolation of the canine inhibin βB subunit gene and characterization of signalling mediated by canine inhibin βB
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犬抑制剂 βB 亚基基因的分离及犬抑制剂 βB 介导的信号表征
DOI：
10.1002/cbf.3664
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发表时间：
2021
影响因子：
3.6
通讯作者：
Murakami Masaru
中科院分区：
文献类型：
--
作者：
Sakota Shotaro;Shimokawa Fumie;Funaba Masayuki;Murakami Masaru
激活素B是一种与β-腺苷酸B亚基同源的二聚体，是性腺功能的调节因子，也是一种脂肪因子。为了阐明激活素B在犬中的作用，我们表征了犬α-胡萝卜素βB基因和由犬α-胡萝卜素βB调节的信号通路。利用5′-和3′-cDNA末端快速扩增（RACE）和RT-PCR技术，我们从狗的卵巢中分离RNA，鉴定了短和长形式的βB基因。在非还原和还原条件下，分别在用含有长型激活素βB基因的质粒转染的HEK 293细胞的培养物上清液中检测到约25和约14 kDa的免疫反应性激活素βB分子，表明激活素B产生和分泌。与人和鼠激活素B相似，犬激活素B刺激报告基因CAGA-luc和Hepcidin-luc的转录，分别受经典激活素/转化生长因子-β（TGF-β）和骨形态发生蛋白（BMP）途径的调节。在ALK 7缺陷型MDCK犬源细胞中未检测到激活素B诱导的CAGA-luc转录;然而，ALK 7的强制表达导致MDCK细胞中激活素B依赖性表达。出乎意料的是，激活素B诱导的BMP途径激活被内源性激活素/TGF-β受体活性的抑制部分阻断。本研究鉴定了实验分离的长型犬骨形成素βB基因，其产生激活素B，该激活素B反式激活BMP-和激活素/TGF-β-调节的基因表达。
Activin B, a homodimer of the inhibin βB subunit, acts as a regulator of gonadal function and as an adipokine. To clarify the role of activin B in dogs, we characterized the canine inhibin βB gene and signalling pathways regulated by the canine inhibin βB. Using 5′‐ and 3′‐rapid amplification of cDNA end (RACE) and RT‐PCR on RNA isolated from the ovary of dogs, we identified short and long forms of the inhibin βB gene. Immunoreactive inhibin βB molecules were detected at ~25 and ~14 kDa under nonreducing and reducing conditions, respectively, in culture supernatants from HEK293 cells transfected with a plasmid containing the long form of the inhibin βB gene, indicating activin B production and secretion. Similar to human and murine activin B, the canine activin B‐stimulated transcriptions of reporter genes, CAGA‐luc and Hepcidin‐luc, regulated by the canonical activin/transforming growth factor‐β (TGF‐β) and bone morphogenetic protein (BMP) pathway, respectively. Activin B‐induced CAGA‐luc transcription was not detected in ALK7‐deficient MDCK canine‐derived cells; however, the forced expression of ALK7 resulted in the activin B‐dependent expression in MDCK cells. Unexpectedly, the activin B‐induced activation of the BMP pathway was partially blocked by the inhibition of endogenous activin/TGF‐β receptor activity. The present study identified an experimentally isolated long form of the canine inhibin βB gene producing activin B that transactivates BMP‐ and activin/TGF‐β‐regulated gene expression.
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影响因子：
3.5
作者：
Kanamori, Yohei;Murakami, Masaru;Funaba, Masayuki
通讯作者：
Funaba, Masayuki
鲤鱼肝脏和肾脏中解偶联蛋白 1 响应环境温度变化的适应性表达。
DOI：
--
发表时间：
2015
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
Masaru Murakami;M. Ohi;Shoko Ishikawa;Mitsuyuki Shirai;Hiroki Horiguchi;Y. Nishino;M. Funaba
通讯作者：
M. Funaba
DOI：
--
发表时间：
2017
期刊：
--
影响因子：
--
作者：
Yohei Kanamori;Masaru Murakami;M. Sugiyama;O. Hashimoto;T. Matsui;M. Funaba
通讯作者：
Yohei Kanamori;Masaru Murakami;M. Sugiyama;O. Hashimoto;T. Matsui;M. Funaba
天然激活素 B 的分离和表征。
DOI：
10.1016/s0021-9258(18)42014-5
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Takanori NakamuraS;Makoto Asashimas;Yuzuru EtolI;Koji Takios;Hideho UchiyamaQ;Naomi MoriyaQ;Takashi AriizumiQ;Takayuki YashiroQ;Kishiko SuginoS;Koiti TitaniSII;Hiromu Sugino
通讯作者：
Hiromu Sugino
影响因子：
3.5
作者：
Kanamori, Yohei;Murakami, Masaru;Funaba, Masayuki
通讯作者：
Funaba, Masayuki