The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets.
The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets.
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DOI：
10.1002/dvdy.21942
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发表时间：
2009-06
影响因子：
2.5
通讯作者：
Wallingford, John B.
中科院分区：
文献类型：
--
作者：
Lee, Chanjae;Le, Minh-Phuong;Wallingford, John B.
在大多数器官系统中发现增厚的上皮片，但其形态发生的机制仍然不清楚。我们在这里表明，蘑菇家族蛋白广泛参与产生增厚的上皮片。通过原位杂交，我们报告的时间和空间的表达模式的四个蘑菇家族成员在非洲爪蟾发育早期从卵母细胞到蝌蚪阶段。Shroom1和2的mRNA是母系表达，而Shroom3和Shroom4是合子转录。在后期发育过程中，所有四种蘑菇家族蛋白在发育中的上皮器官中广泛表达，并且表达蘑菇的上皮细胞被拉长。此外，我们表明，Shroom2的异位表达，像Shroom3，能够增加细胞高度和Shroom2功能的丧失导致神经上皮细胞伸长失败。这些数据表明，蘑菇家族蛋白在几种不同的胚胎上皮组织的形态发生中起着重要作用。
Thickened epithelial sheets are found in most organ systems, but the mechanisms governing their morphogenesis remain poorly defined. We show here that Shroom family proteins are broadly involved in generating thickened epithelial sheets. Through in situ hybridization, we report temporal and spatial expression patterns of the four Shroom family members during early Xenopus development from oocytes to tadpole stages. Shroom1 and 2 mRNAs are maternally expressed, while Shroom3 and Shroom4 are zygotic transcripts. During later development, all four Shroom family proteins are broadly expressed in developing epithelial organs, and the epithelial cells that express Shrooms are elongated. Moreover, we show that ectopic expression of Shroom2, like Shroom3, is able to increase cell height and that loss of Shroom2 function results in a failure of cell elongation in the neural epithelium. These data suggest that Shroom family proteins play an important role in the morphogenesis of several different embryonic epithelial tissues.
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影响因子：
4.8
作者：
Dietz, Megan L.;Bernaciak, Teresa M.;Hildebrand, Jeffrey D.
通讯作者：
Hildebrand, Jeffrey D.
影响因子：
3.5
作者：
SCHIAFFINO, MV;BASSI, MT;BALLABIO, A
通讯作者：
BALLABIO, A
影响因子：
4.8
作者：
Zuckerman, JB;Chen, XY;Smith, PR
通讯作者：
Smith, PR
影响因子：
4
作者：
Hildebrand, JD
通讯作者：
Hildebrand, JD
影响因子：
4.6
作者：
Lee, Chanjae;Scherr, Heather M.;Wallingford, John B.
通讯作者：
Wallingford, John B.