Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling.
Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling.
复制标题
DOI：
10.1038/s41598-021-82926-6
复制
发表时间：
2021-02-19
影响因子：
4.6
通讯作者：
Simonsen AC
中科院分区：
文献类型：
--
作者：
Klenow MB;Heitmann ASB;Nylandsted J;Simonsen AC
质膜修复是真核细胞生存所必需的，它是由钙离子通过膜伤口内流触发的。修复由连续的步骤组成，膜孔的关闭是允许细胞恢复的关键事件，从而确定孔关闭的动力学对于阐明修复机制和作为修复效率的定量处理是重要的。我们在遭受激光损伤的MCF7乳腺癌细胞中进行了钙成像，并结合了一个描述钙时空分布的模型。该模型将孔洞闭合的时间点识别为钙信号最大值时间。细胞数据分析估计闭合时间为：S和S分别使用GCaMP6s-CAAX和GCaMP6s探针。在密封过程中，通过对孔的独立时间推移成像证实了该时间尺度。此外，分析还估计了除钙的特征时间尺度、钙波的穿透深度和扩散系数。孔洞闭合时间的探测成为量化质膜修复的强有力的通用工具
Plasma membrane repair is essential for eukaryotic cell life and is triggered by the influx of calcium through membrane wounds. Repair consists of sequential steps, with closure of the membrane hole being the key event that allows the cell to recover, thus identifying the kinetics of hole closure as important for clarifying repair mechanisms and as a quantitative handle on repair efficiency. We implement calcium imaging in MCF7 breast carcinoma cells subject to laser damage, coupled with a model describing the spatio-temporal calcium distribution. The model identifies the time point of hole closure as the time of maximum calcium signal. Analysis of cell data estimates the closure time as: s and s using GCaMP6s-CAAX and GCaMP6s probes respectively. The timescale was confirmed by independent time-lapse imaging of a hole during sealing. Moreover, the analysis estimates the characteristic time scale of calcium removal, the penetration depth of the calcium wave and the diffusion coefficient. Probing of hole closure times emerges as a strong universal tool for quantification of plasma membrane repair
登录
查看更多内容
影响因子：
16.6
作者：
Boye TL;Maeda K;Pezeshkian W;Sønder SL;Haeger SC;Gerke V;Simonsen AC;Nylandsted J
通讯作者：
Nylandsted J
影响因子：
4
作者：
ALBALDAWI, NF;ABERCROMBIE, RF
通讯作者：
ABERCROMBIE, RF
影响因子：
5.8
作者：
Draeger, Annette;Monastyrskaya, Katia;Babiychuk, Eduard B.
通讯作者：
Babiychuk, Eduard B.
影响因子：
4.3
作者：
Jaiswal, Jyoti K.;Nylandsted, Jesper
通讯作者：
Nylandsted, Jesper
影响因子：
3.7
作者：
Hakobyan D;Gerke V;Heuer A
通讯作者：
Heuer A