Relationship among ovarian follicular status, developmental competence of oocytes, and anti-Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.

Relationship among ovarian follicular status, developmental competence of oocytes, and anti-Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.
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卵泡状态、卵母细胞发育能力和抗 M 抗体之间的关系

DOI:
10.1111/asj.13200
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发表时间:
2019
期刊:
Anim Sci J.
影响因子:
--
通讯作者:
Otoi T.
Otoi T.
中科院分区:
--
文献类型:
--
作者:
Tanihara F;Hirata M;Iizuka S;Sairiki S;Nii M;Nguyen NT;Le QA;Hirano T;Otoi T.

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本研究旨在研究产仔数低于欧洲品种的日本野猪杂交种母猪的卵泡发育、卵母细胞发育能力和血浆抗苗勒氏激素水平。采自两个不同品种的母猪(野生杂交种和大白猪)的卵巢和血浆样本。野生杂交母猪卵巢横切面上次级卵泡和囊泡卵泡的平均数以及来自卵泡的优质卵母细胞数均低于大白母猪(p<0.05)。野生杂交母猪经体外成熟、受精、培养后的优质卵母细胞囊胚期发育率也低于大白母猪(p<0.05)。检测到8.3%的野生杂种母猪和33%的大白母猪血浆AMH水平为0.16 ng/ml。这些结果表明,野生杂交种繁殖力低下的部分原因可能是卵泡数量少、卵母细胞质量好、卵母细胞发育能力低。此外,血浆AMH水平可能支持野生杂交母猪卵巢中低数量的囊泡卵泡。
The aim of this study was to investigate the ovarian follicular development, developmental competence of oocytes, and plasma anti‐Müllerian hormone (AMH) levels of Japanese wild boar crossbred (wild hybrid) gilts, whose litter size is inferior to that of European breeds. Ovary and plasma samples were collected from two different breeds of gilts (wild hybrid and Large White breeds). The ovaries from the wild hybrid gilts had a lower average numbers of secondary follicles and vesicular follicles in ovarian cross‐sections and of good quality oocytes collected from ovarian follicles as compared with those from Large White gilts (p<0.05). The development rate to the blastocyst stage of good quality oocytes after in vitro maturation, fertilization and culture was also lower (p<0.05) in wild hybrid gilts than in Large White gilts. Plasma AMH levels with >0.16 ng/ml were detected in 8.3% of the examined wild hybrid gilts and 33% of the Large White gilts. These results indicate that the low reproductive performance of wild hybrid breed may result in part from low numbers of vesicular follicles and good quality oocytes, and low developmental competence of oocytes. Moreover, plasma AMH levels may support low number of vesicular follicles in ovaries of wild hybrid gilts.
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