Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1.
Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1.
复制标题
DOI：
10.1371/journal.pone.0010042
复制
发表时间：
2010-04-06
期刊：
影响因子：
3.7
通讯作者：
Imhof A
中科院分区：
文献类型：
--
作者：
Boeke J;Regnard C;Cai W;Johansen J;Johansen KM;Becker PB;Imhof A
组蛋白甲基转移酶SU(VAR)3-9在真核细胞异染色质的形成中起重要作用。一些研究表明，在发育过程中，染色质凝集的形成受到高度调控，这表明SU(VAR)3-9‘S的活性也受到调控。然而，到目前为止，还没有关于实现这一目标的机制的报道。由于我们和其他人已经证明SU(VAR)3-9的N-末端对其活性起着重要的作用，我们以含有SU(VAR)3-9 N-末端的GST融合蛋白为诱饵，从果蝇胚胎核提取液中纯化了相互作用伙伴。在已知的与SU(VAR)3-9结合的其他几种蛋白质中，我们分离出了染色体激酶JIL-1，作为一种强相互作用因子。我们证明了SU(VAR)3-9在体外和体内都是JIL-1的底物，并绘制了磷酸化位置图。这些发现可能为观察到的SU(VAR)3-9和JIL-1之间的遗传相互作用提供分子解释。
The histone methyltransferase SU(VAR)3–9 plays an important role in the formation of heterochromatin within the eukaryotic nucleus. Several studies have shown that the formation of condensed chromatin is highly regulated during development, suggesting that SU(VAR)3–9's activity is regulated as well. However, no mechanism by which this may be achieved has been reported so far. As we and others had shown previously that the N-terminus of SU(VAR)3–9 plays an important role for its activity, we purified interaction partners from Drosophila embryo nuclear extract using as bait a GST fusion protein containing the SU(VAR)3–9 N-terminus. Among several other proteins known to bind Su(VAR)3–9 we isolated the chromosomal kinase JIL-1 as a strong interactor. We show that SU(VAR)3–9 is a substrate for JIL-1 in vitro as well as in vivo and map the site of phosphorylation. These findings may provide a molecular explanation for the observed genetic interaction between SU(VAR)3–9 and JIL-1.
登录
查看更多内容
影响因子：
10.5
作者：
Ebert, A;Schotta, G;Reuter, G
通讯作者：
Reuter, G
影响因子：
11.4
作者：
Aagaard, L;Laible, G;Jenuwein, T
通讯作者：
Jenuwein, T
影响因子：
4.6
作者：
Deng, Huai;Bao, Xiaomin;Johansen, Kristen M.
通讯作者：
Johansen, Kristen M.
影响因子：
3.3
作者：
Lerach, Stephanie;Zhang, Weiguo;Johansen, Kristen M.
通讯作者：
Johansen, Kristen M.
影响因子：
2.9
作者：
Chin, HG;Patnaik, D;Pradhan, S
通讯作者：
Pradhan, S