Maize lines expressing RNAi to chromatin remodeling factors are similarly hypersensitive to UV-B radiation but exhibit distinct transcriptome responses.
Maize lines expressing RNAi to chromatin remodeling factors are similarly hypersensitive to UV-B radiation but exhibit distinct transcriptome responses.
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表达RNAi向染色质重塑因子的玉米线对UV-B辐射类似，但表现出明显的转录组响应。
DOI：
10.4161/epi.3.4.6631
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发表时间：
2008-07
期刊：
影响因子：
3.7
通讯作者：
Walbot, Virginia
中科院分区：
文献类型：
--
作者：
Casati, Paula;Walbot, Virginia
针对两个假定的染色质因子（甲基-CpG结合结构域蛋白MBD 101和染色质重塑复合物蛋白CHC 101）的RNAi敲低系在UV-B暴露后表现出相同的表型结果，包括成年叶片的坏死和幼苗死亡。在这里，我们报告说，这些RNAi线表现出显着不同的转录组的变化，在44 K安捷伦寡核苷酸阵列平台上评估相比，彼此和UV-B耐受的非转基因同胞之前和之后的UV-B暴露。成年玉米叶片表达1026000转录本类型在温室生长条件下，8小时的UV-B暴露后，267转录本表现出表达变化的B73控制线。UV-B处理后B73中的这些转录物丰度变化中的大多数在两个RNAi敲低系中没有发现：119种上调的转录物类型和128种下调的转录物类型在B73中被独特地调节。mbd 101 RNAi系显示出比B73或chc 101系（72向上，103向下）更多的系特异性转录物变化（897向上，68向下）。通过功能分析，预测功能受UV-B影响的最大类别的基因是DNA/染色质结合基团。对照和转基因品系中转录因子套件的差异激活是不同转录组谱的可能解释。
RNAi knockdown lines targeting two putative chromatin factors (a methyl-CpG-binding domain protein MBD101 and a chromatin remodeling complex protein CHC101) exhibit identical phenotypic consequences after UV-B exposure including necrosis in adult leaves and seedling death. Here we report that these RNAi lines exhibit substantially different transcriptome changes assessed on a 44K Agilent oligonucleotide array platform compared to each other and to UV-B tolerant non-transgenic siblings both before and after 8 h of UV-B exposure. Adult maize leaves express ∼26,000 transcript types under greenhouse growth conditions; after 8 h of UV-B exposure 267 transcripts exhibit an expression change in the B73 control line. Most of these transcript abundance changes in B73 after UV-B treatment are not found in the two RNAi knockdown lines: 119 up-regulated transcript types and 128 down-regulated types are uniquely modulated in B73. The mbd101 RNAi line shows many more line-specific transcript changes (897 up, 68 down) than either B73 or the chc101line (72 up, 103 down). By functional analysis, the largest category of genes with predicted functions affected by UV-B is the DNA/chromatin binding group. Differential activation of suites of transcription factors in the control and transgenic lines are the likely explanation for the divergent transcriptome profiles.
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影响因子：
12.3
作者：
Casati P;Walbot V
通讯作者：
Walbot V
影响因子：
6.9
作者：
Bergo, E;Segalla, A;Barbato, R
通讯作者：
Barbato, R
影响因子：
1.6
作者：
HAKE, S;WALBOT, V
通讯作者：
WALBOT, V
影响因子：
4.8
作者：
Jeffery, L;Nakielny, S
通讯作者：
Nakielny, S
影响因子：
7.4
作者：
Casati, P;Walbot, V
通讯作者：
Walbot, V