Oligodendrocytes Direct Glycosyl Phosphatidylinositol-anchored Proteins to the Myelin Sheath in Glycosphingolipid-rich Complexes*
Oligodendrocytes Direct Glycosyl Phosphatidylinositol-anchored Proteins to the Myelin Sheath in Glycosphingolipid-rich Complexes*
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少突胶质细胞将糖基磷脂酰肌醇锚定蛋白引导至富含鞘糖脂复合物中的髓鞘*
DOI：
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发表时间：
1997
影响因子：
4.8
通讯作者：
J. Trotter
中科院分区：
文献类型：
--
作者：
E. Krämer;T. Koch;A. Niehaus;J. Trotter
由少突胶质细胞合成的髓鞘隔离中枢神经系统轴突，是质膜的一个特殊亚域，含有有限的蛋白质和脂类。髓鞘富含鞘糖脂和胆固醇，这是糖基磷脂酰肌醇(GPI)锚定蛋白支持的脂质环境，在许多细胞类型的洗涤剂不溶复合体中与这些脂类相关。由于调控少突胶质细胞-神经元相互作用的蛋白在很大程度上是未知的，而且GPI锚定蛋白经常参与细胞-细胞相互作用，我们检测了少突胶质细胞和髓鞘中这些蛋白的表达。少突胶质前体细胞和成熟少突胶质细胞表达类似的GPI锚定蛋白，与大多数少突胶质细胞质膜蛋白不同，这些蛋白聚集在髓鞘中。为了阐明GPI锚定蛋白在髓鞘中表达的机制，我们使用TX-100提取和蔗糖密度梯度分析了细胞和髓鞘中的洗涤剂不可溶复合体。在前体细胞中，GPI锚定的蛋白没有并入洗涤剂不溶的复合体中。相反，从成熟的少突胶质细胞和髓鞘中分离的GPI锚定蛋白被分离为与神经鞘糖脂和胆固醇相关的复合体。这些结果表明，在少突胶质细胞成熟过程中，GPI锚定蛋白与神经鞘糖脂和胆固醇有特定的联系，并建议将这些大分子复合体分选为髓鞘。
The myelin sheath synthesized by oligodendrocytes insulates central nervous system axons and is a specialized subdomain of the plasma membrane, containing a restricted pattern of proteins and lipids. Myelin is enriched in glycosphingolipids and cholesterol, a lipid environment favored by glycosylphosphatidylinositol (GPI)-anchored proteins, which associate with these lipids in detergent-insoluble complexes in many cell types. Since proteins regulating oligodendroglia-neuron interaction are largely unknown and GPI-anchored proteins are often involved in cell-cell interactions, we examined oligodendrocytes and myelin for their expression of these proteins. Oligodendrocyte precursors and maturing oligodendrocytes express a similar pattern of GPI-anchored proteins, which unlike the majority of oligodendrocyte plasma membrane proteins, accumulate in myelin. To elucidate mechanisms underlying the expression of GPI-anchored proteins in myelin, we analyzed detergent-insoluble complexes from cells and myelin using TX-100 extraction and sucrose density gradients. In precursor cells, the GPI-anchored proteins are not incorporated in detergent-insoluble complexes. In contrast, GPI-anchored proteins from maturing oligodendrocytes and from myelin were isolated as complexes associated with glycosphingolipids and cholesterol. These results show a specific association of GPI-anchored proteins with glycosphingolipids and cholesterol during oligodendrocyte maturation and suggest sorting of these macromolecular complexes to myelin.
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用[3H]乙醇胺广泛标记动物细胞的亲水蛋白。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tisdale,EJ;Tartakoff,AM
通讯作者：
Tartakoff,AM
DOI：
10.1073/pnas.91.25.12130
发表时间：
1994-12-06
影响因子：
11.1
作者：
SCHROEDER, R;LONDON, E;BROWN, D
通讯作者：
BROWN, D
成年和幼年大鼠中枢神经系统少突胶质细胞和有髓纤维中 5-核苷酸酶的免疫细胞化学定位。
影响因子：
2.9
作者：
Cammer,W;Sacchi,R;Kahn,S
通讯作者：
Kahn,S
影响因子：
4.4
作者：
MIKOL, DD;RONGNOPARUT, P;STEFANSSON, K
通讯作者：
STEFANSSON, K