Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV).
Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV).
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DOI：
10.1007/s11262-007-0161-5
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发表时间：
2007-12
期刊：
影响因子：
1.6
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Zheng Q;Chen D;Li P;Bi Z;Cao R;Zhou B;Chen P
猪繁殖与呼吸综合征病毒（PRRSV）具有GP5、M和n三种主要结构蛋白，GP5和M蛋白被认为是引起人体对PRRSV感染的体液和细胞免疫应答的重要蛋白，是设计PRRSV生物工程疫苗的理想候选蛋白。在DNA疫苗和腺病毒中表达GP5或M以引起体液和细胞免疫反应的尝试，但很少有论文报道由于GP5和M蛋白在重组病毒中的表达模式不同而引起免疫反应的差异。本文制备了4种表达PRRSV GP5和M蛋白的重组病毒，分别表达了修饰的安卡拉牛痘病毒（MVA）的不同表达模式。在这些重组病毒（rMVAs）中，GP5和M蛋白在同一病毒的MVA中表达，但在两个启动子（rMVA-GP5/M）的控制下，或在一个启动子（rMVA-GP5-M）下作为融合蛋白表达，或分别表达（rMVA-GP5和rMVA-M）。用小鼠模型评价了四种重组病毒的体液和细胞免疫应答。每只小鼠接种5 × 105 TCID50不同的rMVAs，并在3周后增强。初次接种13周后，每周用病毒中和试验法检测各组的中和抗体滴度，评价体液免疫应答。在接种rmva后30,60和90天，检测小鼠脾细胞中γ干扰素（IFN-γ），白细胞介素-2 （IL-2）和白细胞介素-4 （IL-4）的产生，以评估细胞免疫应答。结果表明，rMVA-GP5和rMVA-M不能诱导明显的体液和细胞免疫反应；rMVA-GP5- m接种组的免疫应答优于rMVA-GP5和rMVA-M接种组；然而，接种rMVA-GP5/M的小鼠对prrsv保持最强的细胞应答，并持续增强抗prrsv体液应答。在不同启动子的控制下，在MVA中共同表达PRRSV GP5和M蛋白的策略可能是未来PRRSV疫苗设计的一种有吸引力的方法。
The porcine reproductive and respiratory syndrome virus (PRRSV) has three major structural proteins which designated as GP5, M, and N. Protein GP5 and M have been considered very important to arouse the humoral and cellular immune responses against PRRSV infection and proposed to be the excellent candidate proteins in the design of PRRS bioengineering vaccine. There were some attempts on expressing GP5 or M in DNA vaccine and adenovirus to arouse humoral and cellular immune responses, but few papers have been reported on that the immune response can be difference because of the expression patterns of GP5 and M proteins in the recombinant virus. In this article, four recombinant viruses that expressed GP5 and M proteins of PRRSV in the modified vaccinia virus ankara (MVA) with different expression patterns were made. In these recombinant virus (rMVAs), GP5 and M proteins were expressed in MVA in the same virus but under the control of two promoters (rMVA-GP5/M), or as a fusion protein under one promoter (rMVA-GP5-M), or separately (rMVA-GP5 and rMVA-M). The humoral and cellular immune responses for the four recombinant viruses were evaluated with mouse model. Every mouse was inoculated with 5 × 105 TCID50 of the different rMVAs and boosted 3 weeks later. Neutralizing antibody titers for each group were detected with virus neutralization test assay weekly after the primary inoculation for 13 weeks to evaluate the humoral immune response. The production of gamma interferon (IFN-γ), interleukin-2 (IL-2), and interleukin-4 (IL-4) was detected in splenocytes of rMVA-inoculated mice at 30, 60, and 90 days post inoculation to evaluate the cellular immune response. Results showed that rMVA-GP5 and rMVA-M cannot induce obvious humoral and cellular immune responses; rMVA-GP5-M inoculated group developed better immune responses than rMVA-GP5 and rMVA-M inoculated groups; however, mice inoculated with rMVA-GP5/M maintained the strongest cellular response against PRRS and consistently enhanced the anti-PRRSV humoral responses. The strategy of co-expressing PRRSV GP5 and M protein in MVA under the control of different promoters might be an attractive method for future PRRSV vaccine design.
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影响因子：
5.4
作者：
Ansari, IH;Kwon, B;Pattnaik, AK
通讯作者：
Pattnaik, AK
DOI：
10.1073/pnas.1233578100
发表时间：
2003-08-05
影响因子：
11.1
作者：
Belyakov, IM;Earl, P;Berzofsky, JA
通讯作者：
Berzofsky, JA
影响因子：
5
作者：
Fuertes, LL;Doménech, N;Alonso, F
通讯作者：
Alonso, F
影响因子：
2.7
作者：
Gagnon CA;Lachapelle G;Langelier Y;Massie B;Dea S
通讯作者：
Dea S
影响因子：
3.8
作者：
Blanchard, TJ;Alcamí, A;Smith, GL
通讯作者：
Smith, GL