RNA-seq expression profiling of rat MCAO model following reperfusion Orexin-A.
RNA-seq expression profiling of rat MCAO model following reperfusion Orexin-A.
复制标题
再灌注 Orexin-A 后大鼠 MCAO 模型的 RNA-seq 表达谱
DOI：
10.18632/oncotarget.22995
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发表时间：
2017-12-22
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Wang CM;Pan YY;Liu MH;Cheng BH;Bai B;Chen J
增食欲素-A是一种对脑缺血再灌注损伤具有很强神经保护作用的神经肽，但很少有研究试图阐明其机制。在这里，我们使用RNA测序技术(RNA-seq)对食欲素-A再灌流后的海马区进行了整体基因表达谱分析。RNA-SEQ检测到食欲素-A组与生理盐水对照组(I/R组)相比有649个差异表达基因(DIG)，其中149个上调，500个下调。利用QRT-PCR技术对DEGS进行了鉴定，利用基因本体论(GO)分析了DEGS的分子功能、生物学过程和分子组成，发现了206条与增食欲素-A处理相关的KEGG通路。MAPK、趋化因子和钙信号通路主要参与食欲素-A的神经保护作用。选择Hspb1、Igf2和PTK2B进行功能相互作用分析。结果表明，增食欲素-A改变了海马区的基因表达，从而对I/R损伤具有神经保护作用。这项研究为进一步阐明食欲素-A的分子机制提供了基础。
Orexin-A is a neuropeptide with potent neuroprotective activity towards cerebral ischemia-reperfusion (I/R) injury, but few studies have attempted to elucidate the mechanism. Herein, we performed global gene expression profiling of the hippocampus following reperfusion with Orexin-A using RNA sequencing (RNA-seq). RNA-seq identified 649 differentially expressed genes (DEGs) in the Orexin-A group compared with saline controls (I/R group), of which 149 were up-regulated and 500 were down-regulated. DEGs were confirmed using qRT-PCR, their molecular functions, biological processes and molecular components were explored using Gene Ontology (GO) analysis and 206 KEGG pathways were associated with Orexin-A treatment. MAPK, chemokine and calcium signalling pathways were mainly responsible for the neuroprotective effects of Orexin-A. Hspb1, Igf2 and Ptk2b were selected for functional interaction analysis by GeneMANIA. The results suggest that Orexin-A modifies gene expression in the hippocampus, leading to neuroprotection from I/R injury. The study provides a basis for future elucidation of the molecular mechanisms underlying Orexin-A.
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影响因子：
8
作者：
Meads MB;Fang B;Mathews L;Gemmer J;Nong L;Rosado-Lopez I;Nguyen T;Ring JE;Matsui W;MacLeod AR;Pachter JA;Hazlehurst LA;Koomen JM;Shain KH
通讯作者：
Shain KH
影响因子：
2.2
作者：
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HSP27可保护血脑屏障免受缺血诱导的完整性丧失。
DOI：
10.2174/1871527311312030006
发表时间：
2013-05-01
期刊：
CNS & neurological disorders drug targets
影响因子：
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作者：
Leak RK;Zhang L;Stetler RA;Weng Z;Li P;Atkins GB;Gao Y;Chen J
通讯作者：
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影响因子：
5.8
作者：
Leng, Ning;Dawson, John A.;Kendziorski, Christina
通讯作者：
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作者：
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