Distinct Modes of Cell Death Induced by Different Reactive Oxygen Species
Distinct Modes of Cell Death Induced by Different Reactive Oxygen Species
复制标题
不同活性氧诱导的不同细胞死亡模式
DOI：
--
复制
发表时间：
2002
影响因子：
4.8
通讯作者：
E. Shacter
中科院分区：
文献类型：
--
作者：
R. Englert;E. Shacter
来源于炎性吞噬细胞的氧化剂构成宿主免疫防御系统的关键要素，并且可以通过几种不同机制之一杀死哺乳动物细胞。在这份报告中，我们比较了炎症氧化剂超氧化物，H2 O2和HOCl诱导的人类B淋巴瘤细胞的细胞死亡机制。结果表明，诱导细胞死亡的方式取决于所涉及的氧化剂的性质和细胞被处理的介质。当人伯基特淋巴瘤细胞暴露于超氧阴离子时，作为来自黄嘌呤和黄嘌呤氧化酶的通量产生，细胞通过非凋亡机制（固缩/坏死）死亡，与用试剂H2 O2的团注处理细胞时所见相同。添加超氧化物歧化酶没有影响，而过氧化氢酶是完全保护，表明外源产生的超氧化物完全通过其歧化到H2 O2杀死细胞。相反，在培养基中用试剂HOCl处理的细胞主要通过凋亡死亡。HOCl诱导的细胞凋亡是由培养基中产生的氨酰氯胺介导的，并且可以通过用牛磺酸氯胺或用由修饰的Lys或Arg产生的长寿命氯胺处理细胞来模拟。结果表明，在O2-和H2 O2是形成的主要氧化剂的生理环境中，细胞死亡的主要形式可能是坏死性的，而在产生HOCl的炎症条件下，凋亡性细胞死亡可能占主导地位。
Oxidants derived from inflammatory phagocytes compose a key element of the host immune defense system and can kill mammalian cells by one of several different mechanisms. In this report, we compare mechanisms of cell death induced in human B lymphoma cells by the inflammatory oxidants superoxide, H2O2, and HOCl. The results indicate that the mode of cell death induced depends on the nature of the oxidant involved and the medium in which the cells are treated. When human Burkitt's lymphoma cells are exposed to superoxide anion, generated as a flux from xanthine and xanthine oxidase, the cells die by a non-apoptotic mechanism (pyknosis/necrosis) identical to that seen when cells are treated with a bolus of reagent H2O2. Addition of superoxide dismutase has no effect, whereas catalase is completely protective, indicating that exogenously generated superoxide kills cells entirely through its dismutation into H2O2. In contrast, cells treated in culture media with reagent HOCl die largely by apoptosis. HOCl-induced apoptosis is mediated by aminoacyl chloramines generated in the culture media and can be mimicked by treatment of cells with taurine chloramine or with long lived chloramines generated from modified Lys or Arg. The results suggest that in a physiological milieu in which O 2 − and H2O2 are the main oxidants being formed, the principal form of cell death may be necrotic, and under inflammatory conditions in which HOCl is generated, apoptotic cell death may predominate.
登录
查看更多内容
牛磺酸在中性粒细胞对红细胞的体外氧化毒性中作用的证据。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thomas,EL;Grisham,MB;Melton,DF;Jefferson,MM
通讯作者：
Jefferson,MM
DOI：
10.1172/jci111544
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Test,ST;Lampert,MB;Ossanna,PJ;Thoene,JG;Weiss,SJ
通讯作者：
Weiss,SJ
影响因子：
--
作者：
E. Thomas;M. Grisham;M. Jefferson
通讯作者：
E. Thomas;M. Grisham;M. Jefferson
影响因子：
--
作者：
DAVIES, JMS;HORWITZ, DA;DAVIES, KJA
通讯作者：
DAVIES, KJA
影响因子：
15.9
作者：
SPRAGG, RG;HINSHAW, DB;COCHRANE, CG
通讯作者：
COCHRANE, CG