Dynein light chain 1 functions in somatic cyst cells regulate spermatogonial divisions in Drosophila.
Dynein light chain 1 functions in somatic cyst cells regulate spermatogonial divisions in Drosophila.
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DOI：
10.1038/srep00173
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发表时间：
2011
影响因子：
4.6
通讯作者：
Ray, Krishanu
中科院分区：
文献类型：
--
作者：
Joti, Prakash;Ghosh-Roy, Anindya;Ray, Krishanu
干细胞的后代在分化前经常经历转运扩增分裂。在果蝇中，精原体前体在由两个体细胞起源的囊肿细胞形成的外壳内分裂四次，然后分化为精母细胞。虽然已知生殖系和囊肿细胞的内在因子调节这些分裂，但其机制细节尚不清楚。在这里，我们发现囊肿细胞中动力蛋白轻链-1 （DDLC1/LC8）的缺失消除了精原细胞中弹袋（bam）的表达，导致果蝇睾丸性腺细胞增生。表型主要由Dhc64C（细胞质动力蛋白）和didum（肌球蛋白V）功能缺失等位基因增强。囊肿细胞中DDLC1或Myosin V的缺失也会影响其分化。此外，囊肿细胞特异性缺失的ddlc1破坏了Armadillo、DE-cadherin和整合素-βPS在囊肿中的定位。综上所述，这些结果表明，Dynein和Myosin V活性以及独立的DDLC1功能在囊肿细胞中组织了调节精原细胞增殖和分化的体细胞微环境。
Stem cell progeny often undergo transit amplifying divisions before differentiation. In Drosophila, a spermatogonial precursor divides four times within an enclosure formed by two somatic-origin cyst cells, before differentiating into spermatocytes. Although germline and cyst cell-intrinsic factors are known to regulate these divisions, the mechanistic details are unclear. Here, we show that loss of dynein-light-chain-1 (DDLC1/LC8) in the cyst cells eliminates bag-of-marbles (bam) expression in spermatogonia, causing gonial cell hyperplasia in Drosophila testis. The phenotype is dominantly enhanced by Dhc64C (cytoplasmic Dynein) and didum (Myosin V) loss-of-function alleles. Loss of DDLC1 or Myosin V in the cyst cells also affects their differentiation. Furthermore, cyst cell-specific loss of ddlc1 disrupts Armadillo, DE-cadherin and Integrin-βPS localizations in the cyst. Together, these results suggest that Dynein and Myosin V activities, and independent DDLC1 functions in the cyst cells organize the somatic microenvironment that regulates spermatogonial proliferation and differentiation.
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