JPHYD Inhibits miR-21-5p/Smad7-Mediated Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma Cells.
JPHYD Inhibits miR-21-5p/Smad7-Mediated Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma Cells.
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JPHYD 抑制 miR-21-5p/Smad7 介导的肝细胞癌细胞上皮-间质转化
作者：
有研究表明健脾化瘀汤具有抑制肝癌细胞生长的作用，但其作用机制目前尚不清楚。 我们以肝癌细胞为体外细胞模型，以异种移植瘤为体内模型，评估JPHYD的作用。CCK 8、EdU、伤口愈合和transwell试验用于评估肝细胞癌（HCC）细胞系HepG 2和MHCC 97 H的细胞生长、迁移和侵袭。Western blot检测E-cadherin、Smad 7、N-cadherin、Snail、Smad 3、Vimentin和Zeb 1蛋白表达水平。采用qRT-PCR方法检测miR-21- 5 p在临床肝癌组织、HepG 2和MHCC 97 H细胞中的表达。进行动物致瘤实验和体内成像实验以评估体外实验的结果。 JPHYD可抑制肝癌细胞的增殖、侵袭和迁移，降低N-cadherin、Snail、Vimentin、Smad 3和Zeb 1的表达，升高E-cadherin和Smad 7的表达。肝癌组织中miR-21- 5 p表达增强，Smad 7蛋白表达减弱。体内实验也显示miR-21- 5 p能促进肝癌细胞的迁移。JPHYD降低miR-21- 5 p表达。在动物研究中也发现了同样的结果。 我们的结果表明，JPHYD抑制上皮间质转化通过增加Smad 7的表达和抑制miR-21- 5 p。因此，阻断EMT的发生和发展可能是JPHYD抗肝癌作用的新机制。
Studies have shown that Jianpi Huayu Decoction (JPHYD) can inhibit the growth of hepatocellular carcinoma cells, but the mechanism of its effect was not clear at present. We assessed the effect of JPHYD using liver cancer cells as in vitro cell model and xenograft tumor as in vivo model. CCK8, EdU, wound-healing, and transwell assays were performed to assess the cell growth, migration, and invasion of hepatocellular carcinoma (HCC) cell lines HepG2 and MHCC97H. Western blot assay was performed to observe the protein level of E-cadherin, Smad7, N-cadherin, Snail, Smad3, Vimentin, and Zeb1. qRT-PCR assay was used to observe the expression of miR-21-5p in clinical liver cancer tissue samples and in HepG2 and MHCC97H cells. Animal tumorigenesis experiments and in vivo imaging experiments were performed to assess the results of in vitro experiments. We found that JPHYD could inhibit the proliferation, invasion, and migration of hepatocellular carcinoma cells and JPHYD decreased the level of N-cadherin, Snail, Vimentin, Smad3, and Zeb1 and increased E-cadherin and Smad7 proteins. The expression of miR-21-5p was increased while that protein of Smad7 was decreased in HCC tissues. The vivo experiments also showed that miR-21-5p could promote the migration of HCC cells. JPHYD decreased miR-21-5p expression. The same results have been found in animal studies. Our results indicated that JPHYD inhibited epithelial-mesenchymal transition by increasing Smad7 expression and inhibiting miR-21-5p. Therefore, blocking the occurrence and development of EMT may be a new mechanism of JPHYD's anti-liver cancer effect.
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影响因子：
--
作者：
Zhong, Chong;Li, Hui-Dong;Guo, Rong-Ping
通讯作者：
Guo, Rong-Ping
DOI：
10.1016/s0140-6736(17)33326-3
发表时间：
2018-03-17
期刊：
Lancet (London, England)
影响因子：
--
作者：
Allemani C;Matsuda T;Di Carlo V;Harewood R;Matz M;Nikšić M;Bonaventure A;Valkov M;Johnson CJ;Estève J;Ogunbiyi OJ;Azevedo E Silva G;Chen WQ;Eser S;Engholm G;Stiller CA;Monnereau A;Woods RR;Visser O;Lim GH;Aitken J;Weir HK;Coleman MP;CONCORD Working Group
通讯作者：
CONCORD Working Group
影响因子：
8.8
作者：
Ning, Xia;Wang, Yue;Sang, Nan
通讯作者：
Sang, Nan
健脾化瘀汤通过靶向骨髓源性抑制细胞减轻 H-22 肝细胞癌小鼠的免疫抑制状态
影响因子：
5.6
作者：
Xie, Yingjie;Zhang, Yuan;Wang, Changjun
通讯作者：
Wang, Changjun
影响因子：
64.5
作者：
Lambert AW;Pattabiraman DR;Weinberg RA
通讯作者：
Weinberg RA