Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases.
Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases.
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DOI：
10.1038/s41598-018-29112-3
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发表时间：
2018-07-16
影响因子：
4.6
通讯作者：
Hayashi M
中科院分区：
文献类型：
--
作者：
Okamoto M;Takahashi Y;Komichi S;Cooper PR;Hayashi M
牙本质主要由富含有机基质的羟基磷灰石晶体组成。有机基质包含胶原结构组分，其中隔离了多种生物活性分子。在龋病进展过程中，牙本质被各种来源的酸和酶降解，这些酸和酶可以释放对牙本质-牙髓复合体具有潜在修复活性的生物活性分子。虽然这些分子在其他组织中的修复活性是已知的，但它们的生物学效应与牙本质-牙髓复合体疾病期间的降解事件的关系尚不清楚。本研究旨在探讨基质金属蛋白酶（matrix metalloproteinases，MMPs）部分消化牙本质基质成分（dentin matrix components，DMCs）在牙本质-牙髓复合体创伤愈合过程中的作用。最初从健康牙本质中分离DMCs，并用重组MMP处理。随后，在体外和体内研究了它们对牙髓细胞行为的影响。消化后的DMCs在体外可调节一系列牙髓细胞功能。此外，部分消化与MMP-20的DMCs刺激三级牙本质形成在体内，表现出更规则的管状结构比处理与其他MMP诱导。我们的研究结果表明，MMP-20可能是特别有效的刺激伤口愈合的牙本质牙髓复合体。
Dentin is primarily composed of hydroxyapatite crystals within a rich organic matrix. The organic matrix comprises collagenous structural components, within which a variety of bioactive molecules are sequestered. During caries progression, dentin is degraded by acids and enzymes derived from various sources, which can release bioactive molecules with potential reparative activity towards the dentin-pulp complex. While these molecules’ repair activities in other tissues are already known, their biological effects are unclear in relation to degradation events during disease in the dentin-pulp complex. This study was undertaken to investigate the effects of dentin matrix components (DMCs) that are partially digested by matrix metalloproteinases (MMPs) in vitro and in vivo during wound healing of the dentin-pulp complex. DMCs were initially isolated from healthy dentin and treated with recombinant MMPs. Subsequently, their effects on the behaviour of primary pulp cells were investigated in vitro and in vivo. Digested DMCs modulated a range of pulp cell functions in vitro. In addition, DMCs partially digested with MMP-20 stimulated tertiary dentin formation in vivo, which exhibited a more regular tubular structure than that induced by treatment with other MMPs. Our results indicate that MMP-20 may be especially effective in stimulating wound healing of the dentin-pulp complex.
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影响因子：
3.1
作者：
Chaussain C;Eapen AS;Huet E;Floris C;Ravindran S;Hao J;Menashi S;George A
通讯作者：
George A
影响因子：
4.4
作者：
Adair-Kirk, TL;Atkinson, JJ;Senior, RM
通讯作者：
Senior, RM
影响因子：
4.8
作者：
Caterina, JJ;Skobe, Z;Bartlett, JD
通讯作者：
Bartlett, JD
影响因子：
3
作者：
Hedenbjork-Lager, Anders;Hamberg, Kristina;Ericson, Dan
通讯作者：
Ericson, Dan
影响因子：
3.7
作者：
Ishimoto K;Hayano S;Yanagita T;Kurosaka H;Kawanabe N;Itoh S;Ono M;Kuboki T;Kamioka H;Yamashiro T
通讯作者：
Yamashiro T