Topical application of lithium chloride on the pulp induces dentin regeneration.
Topical application of lithium chloride on the pulp induces dentin regeneration.
复制标题
DOI：
10.1371/journal.pone.0121938
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yamashiro T
中科院分区：
文献类型：
--
作者：
Ishimoto K;Hayano S;Yanagita T;Kurosaka H;Kawanabe N;Itoh S;Ono M;Kuboki T;Kamioka H;Yamashiro T
我们在此描述了一种新的牙本质再生程序，它模仿了自然界中牙齿发育的生物过程。典型的Wnt信号通路是牙本质唾液磷蛋白（Dspp）表达的重要调节因子。我们的方法模拟了自然界中潜在的牙齿发育的生物过程，并专注于激活典型的Wnt信号来触发牙本质形成的自然过程。牙本质的冠状部分和下面的牙髓被从第一磨牙上移除。我们将典型Wnt信号激活剂氯化锂（LiCl）应用于切除的牙髓表面，以实现周围牙髓细胞向成牙髓细胞的转分化。显微ct和显微分析表明，局部应用LiCl可诱导牙本质修复，包括形成完整的牙本质桥。licl诱导的牙本质是管状牙本质，其牙髓细胞不像初级牙本质那样嵌入基质中。相比之下，仅用材料载体盖髓治疗的对照组没有诱导牙本质桥，尽管在离牙髓暴露部位相对较深的位置诱导了未形成管状的骨牙本质蛋白。我们还评估了LiCl对体外成牙细胞分化的影响。在mDP成牙细胞系中，LiCl激活Dspp、Axin2和Kallikrein 4 (Klk4) mRNA表达，下调骨桥蛋白（Osteopontin, Osp）表达。这些结果为利用LiCl作为牙本质再生的新型封盖材料进行牙本质仿生再生提供了科学依据。
We herein describe a novel procedure for dentin regeneration that mimics the biological processes of tooth development in nature. The canonical Wnt signaling pathway is an important regulator of the Dentin sialophosphoprotein (Dspp) expression. Our approach mimics the biological processes underlying tooth development in nature and focuses on the activation of canonical Wnt signaling to trigger the natural process of dentinogenesis. The coronal portion of the dentin and the underlying pulp was removed from the first molars. We applied lithium chloride (LiCl), an activator of canonical Wnt signaling, on the amputated pulp surface to achieve transdifferentiation toward odontoblasts from the surrounding pulpal cells. MicroCT and microscopic analyses demonstrated that the topical application of LiCl induced dentin repair, including the formation of a complete dentin bridge. LiCl-induced dentin is a tubular dentin in which the pulp cells are not embedded within the matrix, as in primary dentin. In contrast, a dentin bridge was not induced in the control group treated with pulp capping with material carriers alone, although osteodentin without tubular formation was induced at a comparatively deeper position from the pulp exposure site. We also evaluated the influence of LiCl on differentiation toward odontoblasts in vitro. In the mDP odontoblast cell line, LiCl activated the mRNA expression of Dspp, Axin2 and Kallikrein 4 (Klk4) and downregulated the Osteopontin (Osp) expression. These results provide a scientific basis for the biomimetic regeneration of dentin using LiCl as a new capping material to activate dentine regeneration.
登录
查看更多内容
影响因子：
9.8
作者：
Lammi, L;Arte, S;Nieminen, P
通讯作者：
Nieminen, P
影响因子：
5
作者：
Cruz, EV;Kota, K;Hoshino, E
通讯作者：
Hoshino, E
影响因子：
7.6
作者：
Nagano, T;Oida, S;Fukae, M
通讯作者：
Fukae, M
影响因子：
2.5
作者：
Lohi, Mahtab;Tucker, Abigail S.;Sharpe, Paul T.
通讯作者：
Sharpe, Paul T.
影响因子：
9.8
作者：
Adaimy, Lynn;Chouery, Eliane;Megarbane, Andre
通讯作者：
Megarbane, Andre