Regional localization of virus in the central nervous system of mice persistently infected with murine coronavirus JHM.
Regional localization of virus in the central nervous system of mice persistently infected with murine coronavirus JHM.
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DOI：
10.1016/0042-6822(88)90503-x
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发表时间：
1988-10
期刊：
影响因子：
3.7
通讯作者：
Moore S
中科院分区：
文献类型：
--
作者：
Perlman S;Jacobsen G;Moore S
用小鼠肝炎病毒株JHM（MHV-JHM）感染的哺乳C57 BU 6小鼠发展为致死性急性脑脊髓炎或迟发性脱髓鞘疾病，这取决于它们是否由未免疫或免疫的母鼠喂养。为了确定病毒特异性RNA的定位，用35 S标记的反义RNA探针对感染和未感染小鼠的脑连续切片进行退火，并通过胶片放射自显影进行分析。在急性脑脊髓炎小鼠中，病毒RNA存在于中脑、下丘脑、海马、基底神经节、皮质下白色物质和丘脑中。在所有迟发性脱髓鞘性脑脊髓炎小鼠的脊髓中检测到病毒RNA，但在这些小鼠的大脑中分布成三种不同的模式，即使所有小鼠都患有相同的临床疾病。在第一组中，仅在脑干中检测到病毒RNA。在第二组中，在脑干、丘脑和大脑灰质中检测到病毒RNA。这种分布与病毒沿沿着连接大脑这些部分的明确的神经束传播一致。在第三组中，在脑干和视交叉附近的几个白色物质束中都可以检测到病毒RNA。这种分布与病毒通过细胞外途径从一条白色道传播到物理上接近但不属于相同途径的其他道一致。这些结果表明，MHV-JHM通过中枢神经系统传播，既可以沿着明确的神经元通路传播，也可以从相邻结构传播，但也表明病毒优先在有限数量的大脑区域复制。原位杂交技术结合胶片放射自显影技术可广泛用于分析病毒在感染器官内的宏观运动。
Suckling C57BU6 mice infected with mouse hepatitis virus strain JHM (MHV-JHM) develop either a fatal acute encephalomyelitis or a late onset demyelinating disease, depending on whether they are nursed by unimmunized or immunized dams. To determine the localization of virus-specific RNA, serial sections of brains from infected and uninfected mice were annealed with a 35S-labeled antisense RNA probe and analyzed by film autoradiography. In the mice with acute encephalomyelitis, viral RNA was present in the mesencephalon, hypothalamus, hippocampus, basal ganglia, subcortical white matter, and thalamus. Viral RNA was detected in the spinal cords of all mice with the late onset, demyelinating encephalomyelitis, but was distributed into three different patterns in the brains of these mice, even though all had the same clinical disease. In the first group, viral RNA was detected only in the brainstem. In the second group, viral RNA was detected in the brainstem, thalamus, and cerebral grey matter. This distribution was consistent with viral spread along well-defined tracts connecting these parts of the brain. In the third group, viral RNA could be detected both in the brainstem and in several white matter tracts within close physical proximity to the optic chiasm. This distribution was consistent with viral spread by an extracellular route from one white matter tract to other tracts which were physically close, but which were not part of the same pathways. These results suggest that MHV-JHM spreads through the central nervous system both along well-defined neuronal pathways and by spread from contiguous structures, but also suggest that viral replicates preferentially in a limited number of areas of the brain. The technique of in situ hybridization with film autoradiography should be generally useful for analyzing macroscopic movements of virus within infected organs.
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DOI：
10.1084/jem.90.3.195
发表时间：
1949-08-31
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bailey OT;Pappenheimer AM;Cheever FS;Daniels JB
通讯作者：
Daniels JB
影响因子：
--
作者：
WEINER, LP
通讯作者：
WEINER, LP
影响因子：
5.4
作者：
FLEMING, JO;TROUSDALE, MD;WEINER, LP
通讯作者：
WEINER, LP
影响因子：
5.4
作者：
BUDZILOWICZ, CJ;WILCZYNSKI, SP;WEISS, SR
通讯作者：
WEISS, SR
影响因子：
5
作者：
PERLMAN, S;RIES, D;STOLTZFUS, CM
通讯作者：
STOLTZFUS, CM