Cloning and increased expression with fructose feeding of rat jejunal GLUT5.
Cloning and increased expression with fructose feeding of rat jejunal GLUT5.
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大鼠空肠 GLUT5 的克隆和果糖喂养增加表达。
DOI：
10.1210/endo.133.5.8404647
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发表时间：
1993
期刊：
影响因子：
4.8
通讯作者：
Y. Oka
中科院分区：
文献类型：
--
作者：
K. Inukai;T. Asano;H. Katagiri;H. Ishihara;M. Anai;Y. Fukushima;K. Tsukuda;M. Kikuchi;Y. Yazaki;Y. Oka
我们用人GLUT 5 cDNA片段作为探针，从大鼠空肠cDNA文库中分离到一个克隆。该克隆的编码区与人GLUT 5具有80%的核苷酸和81%的氨基酸同一性，因此被称为大鼠GLUT 5 cDNA。大鼠GLUT 5 mRNA的组织分布与人GLUT 5非常相似，在空肠中的水平最高，但在脂肪、肌肉或睾丸中未检测到北方印迹分析。针对大鼠GLUT 5蛋白的C-末端结构域产生的抗肽抗体特异性地识别大鼠空肠蛋白，其在免疫印迹上的表观摩尔重量为60，000。与喂食标准大鼠食物的大鼠相比，喂食富含果糖饮食（50%，wt/wt）3天的大鼠空肠中GLUT 5 mRNA和蛋白质的量分别增加了2.5倍和6倍，而喂食富含淀粉饮食（50%，wt/wt）的大鼠空肠中GLUT 5 mRNA和蛋白质的量没有改变。同样，GLUT 5的mRNA和蛋白质在空肠增加5倍和8倍，分别在15天的果糖喂养。因此，果糖吸收的增加上调空肠中的GLUT 5表达。这些结果与GLUT 5在小肠果糖吸收中起主要作用的观点一致。
We have isolated a clone from the rat jejunum cDNA library using a fragment of human GLUT5 cDNA as a probe. The coding region of this clone shares 80% nucleotide and 81% amino acid identity with human GLUT5 and is thus termed rat GLUT5 cDNA. Rat GLUT5 mRNA exhibited a tissue distribution very similar to that of human GLUT5, with the highest levels in the jejunum, but was not detected in fat, muscle, or testis on Northern blot analysis. The antipeptide antibody raised against the C-terminal domain of rat GLUT5 protein specifically recognized a rat jejunal protein with an apparent mol wt of 60,000 on immunoblots. The amount of GLUT5 mRNA and protein in the jejuni of rats fed a fructose-enriched diet (50%, wt/wt) for 3 days were increased 2.5- and 6-fold, respectively, compared to those of rats fed standard rat chow, whereas those of rats fed a starch-enriched diet (50%, wt/wt) were not altered. Similarly, GLUT5 mRNA and protein in the jejunum were increased 5- and 8-fold, respectively, after 15 days of fructose feeding. Thus, an increase in fructose absorption up-regulates GLUT5 expression in the jejunum. These results are consistent with the notion that GLUT5 plays a major role in fructose absorption in the small intestine.
影响因子：
29.4
作者：
FUJISAWA, T;RIBY, J;KRETCHMER, N
通讯作者：
KRETCHMER, N
DOI：
10.1016/s0021-9258(18)42067-4
发表时间：
1992-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Burant;J. Takeda;É. Brot-Laroche;G. Bell;N. Davidson
通讯作者：
C. Burant;J. Takeda;É. Brot-Laroche;G. Bell;N. Davidson
果糖诱导大鼠高甘油三酯血症的机制。
DOI：
10.1042/bj2820753
发表时间：
1992
期刊：
The Biochemical journal
影响因子：
--
作者：
Park,OJ;Cesar,D;Faix,D;Wu,K;Shackleton,CH;Hellerstein,MK
通讯作者：
Hellerstein,MK