Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
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甜橙柑橘 MYB 转录因子家族的全基因组分类以及进化和表达分析
DOI：
10.1371/journal.pone.0112375
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang JZ
中科院分区：
文献类型：
--
作者：
Hou XJ;Li SB;Liu SR;Hu CG;Zhang JZ
MYB家族基因广泛分布于植物中，是参与植物各种发育过程和防御反应的最大转录因子之一。迄今为止，很少有MYB基因和表达谱已报告柑橘。在这里，我们描述和分类177个成员的甜橙子MYB基因（CsMYB）家族的基因组基因结构和相似性，他们假定的拟南芥直向同源物。根据这些分析，这些CsMYB被分为四组（4 R-MYB、3R-MYB、2 R-MYB和1 R-MYB）。基因结构分析表明，1 R-MYB基因相对于2 R-MYB基因具有更多的内含子。对它们的染色体定位的调查显示，这些CsMYBs分布在9条染色体上。甜橙子中MYB基因的数目相对较少，推测可能与基因启动子和非编码转录区插入重复序列有关。对CsMYBs和拟南芥的比较研究表明，CsMYBs具有较少的基因重复事件。表达分析表明，MYB基因家族在甜橙子发育过程中具有广泛的表达谱，并在发育和胁迫反应中发挥重要作用。此外，337个新的推定微卫星侧翼序列足以引物设计也确定了从177 CsMYBs。这些结果为柑橘MYB基因的克隆和功能分析提供了参考。
MYB family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. To date, few MYB genes and little expression profiling have been reported for citrus. Here, we describe and classify 177 members of the sweet orange MYB gene (CsMYB) family in terms of their genomic gene structures and similarity to their putative Arabidopsis orthologs. According to these analyses, these CsMYBs were categorized into four groups (4R-MYB, 3R-MYB, 2R-MYB and 1R-MYB). Gene structure analysis revealed that 1R-MYB genes possess relatively more introns as compared with 2R-MYB genes. Investigation of their chromosomal localizations revealed that these CsMYBs are distributed across nine chromosomes. Sweet orange includes a relatively small number of MYB genes compared with the 198 members in Arabidopsis, presumably due to a paralog reduction related to repetitive sequence insertion into promoter and non-coding transcribed region of the genes. Comparative studies of CsMYBs and Arabidopsis showed that CsMYBs had fewer gene duplication events. Expression analysis revealed that the MYB gene family has a wide expression profile in sweet orange development and plays important roles in development and stress responses. In addition, 337 new putative microsatellites with flanking sequences sufficient for primer design were also identified from the 177 CsMYBs. These results provide a useful reference for the selection of candidate MYB genes for cloning and further functional analysis forcitrus.
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影响因子：
14.9
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