Prospective assessment of the gastroesophageal microbiome in VLBW neonates.
Prospective assessment of the gastroesophageal microbiome in VLBW neonates.
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DOI：
10.1186/1471-2431-13-49
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发表时间：
2013-04-05
期刊：
影响因子：
2.4
通讯作者：
Schröder I
中科院分区：
文献类型：
--
作者：
Milisavljevic V;Garg M;Vuletic I;Miller JF;Kim L;Cunningham TD;Schröder I
住院早产儿的远端胃肠道微生物区系已被确定为不同于健康足月新生儿的微生物区系；近端胃肠道微生物区系，更具体地说是胃食道定植，尚未被系统地解决。我们前瞻性地评估了极低出生体重儿胃食道部分的早期定植。这项研究涉及12名住进三级NICU的婴儿，胎龄(GA)27+/−0.5周，出生体重1105+/−77克。用16S rDNA分析方法对出生后头28天无菌采集的吸液进行了胃食道微生物区系的评估。12例新生儿中有9例检出细菌。出生后第1周以解脲支原体为主，但以葡萄球菌为主。到第四周，革兰氏(−)细菌数量增加，占细菌总数的50%。在大多数新生儿中存在氟米米特，并持续了4周，占测序克隆的近一半。值得注意的是，只发现了两种不同的表皮葡萄球菌，表明是从环境中获得的。在我们的新生儿中，食道和胃环境从出生时相对不育转变为被系统发育多样化的低个体复杂性的微生物区系定植。到第四周时，我们发现细菌优势种为菲米米特菌和变形杆菌。来自两个门(cons和革兰氏(−)生物)的细菌被强烈地牵连为医院获得性感染的原因。对防止来自医院环境的潜在致病微生物和致病微生物定居的措施进行评估可能是有必要的，并可能提出提高新生儿护理质量的新方法。
The distal GI microbiota of hospitalized preterm neonates has been established to be unique from that of healthy full-term infants; the proximal GI, more specifically gastroesophageal colonization has not been systematically addressed. We prospectively evaluated early colonization of gastroesophageal portion of the GI tract of VLBW infants. This study involved 12 infants admitted to a level III NICU with gestational age (GA) 27 +/− 0.5 weeks and birth weight 1105 +/− 77 grams. The gastroesophageal microbial flora was evaluated using 16S rDNA analysis of aspirates collected in a sterile manner during the first 28 days of life. Bacteria were detected in 9 of the 12 neonates. Ureaplasma was the dominant species in the first week of life, however, staphylococci were the predominant bacteria overall. By the fourth week, Gram (−) bacteria increased in abundance to account for 50% of the total organisms. Firmicutes were present in the majority of the neonates and persisted throughout the 4 weeks comprising nearly half of the sequenced clones. Noticeably, only two distinct species of Staphylococcus epidermidis were found, suggesting acquisition from the environment. In our neonates, the esophagus and stomach environment changed from being relatively sterile at birth to becoming colonized by a phylogenetically diverse microbiota of low individual complexity. By the fourth week, we found predominance of Firmicutes and Proteobacteria. Bacteria from both phyla (CONS and Gram (−) organisms) are strongly implicated as causes of hospital-acquired infections (HAI). Evaluation of the measures preventing colonization with potentially pathogenic and pathogenic microorganisms from the hospital environment may be warranted and may suggest novel approaches to improving quality in neonatal care.
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影响因子：
4.2
作者：
Duffy, LC
通讯作者：
Duffy, LC
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作者：
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通讯作者：
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