Hepatitis B splice-generated protein antibodies in Syrian chronic hepatitis B patients: incidence and significance.
Hepatitis B splice-generated protein antibodies in Syrian chronic hepatitis B patients: incidence and significance.
复制标题
DOI：
10.5812/hepatmon.13166
复制
发表时间：
2014-04
影响因子：
0.6
通讯作者：
Monem F
中科院分区：
文献类型：
--
作者：
Al-Hanafi N;Monem F
以前的研究表明，乙肝剪接生成蛋白(HBSP)在表达时，参与了乙肝病毒感染的发病机制。我们的目的是评估一组叙利亚慢性乙型肝炎患者中抗-HBSP的发生率及其与几个乙肝病毒感染参数的关系。80例治疗前HBs Ag阳性的成人慢性乙肝患者的血清纳入我们的前瞻性靶向性研究。肝功能、病毒学和组织学检测结果从患者的医疗档案中获得。根据从叙利亚患者血清中获得的乙肝病毒基因组序列，设计了一个20聚体HBSP衍生肽的三个变体(GenBank登录号：JN257148-JN257217)。将合成的多肽包被微板孔板，用优化的间接酶联免疫吸附试验(ELISA)检测抗HBSP抗体。当样本的光密度(OD)值高于至少一种多肽变体的截止值时，被认为是阳性。80例CHB患者中有7例(9%)抗HBSP抗体阳性。HBeAg阳性和HBeAg阴性患者的平均OD值差异无统计学意义(P>；0.05)。OD值与ALT、AST呈弱正相关(P<；0.05)，与肝活检分期呈弱至中度正相关(P<；0.05)。病毒载量和肝活检分级等级之间无显著相关性(P>；0.05)。我们介绍了一种抗HBSP抗体的酶联免疫吸附试验，专为当地流行的乙肝病毒株而设计。观察到抗-HBSP与肝纤维化分期的相关性，而不考虑病毒复制和肝脏炎症，提示抗-HBSP抗体可能是乙肝病毒相关性肝纤维化的指标。
Previous studies have suggested hepatitis B splice-generated protein (HBSP), when expressed, is involved in the pathogenesis of HBV infection. We aimed to evaluate anti-HBSP incidence and association with several HBV infection parameters in a group of Syrian chronic hepatitis B patients. Eighty treatment-naïve HBsAg-positive adult chronic hepatitis B patients' sera were included in our prospective targeted study. Liver function, virological and histological tests results were obtained from patients’ medical files. Three variants of a 20-mer HBSP-derived peptide were designed based on HBV genome sequences obtained from Syrian patients' sera (GenBank Accession No. JN257148-JN257217). Microtiter plate wells were coated with the synthetic peptides and used to detect anti-HBSP antibodies by an optimized indirect enzyme-linked immunosorbent assay (ELISA). Samples were considered positive when showed optical density (OD) values higher than the cut-off value for at least one peptide variant. Seven out of eighty (9%) CHB patients were positive for anti-HBSP antibodies. Mean OD values were not significantly different between HBeAg-positive and -negative patients (P > 0.05). OD values showed weak positive correlation with ALT and AST values (P < 0.05), and weak to moderate positive correlation with liver biopsy staging ranks (P < 0.05). No significant correlation was revealed with viral load values or liver biopsy grading ranks (P > 0.05). We introduced an anti-HBSP antibodies ELISA, designed for locally circulating HBV strains. Correlation observed of Anti-HBSP with liver fibrosis staging regardless of viral replication and liver inflammation suggests anti-HBSP antibodies as possible indicator for HBV-associated liver fibrosis.
登录
查看更多内容
影响因子：
13.5
作者：
Preiss, Scott;Littlejohn, Margaret;Revill, Peter A.
通讯作者：
Revill, Peter A.
影响因子：
15.9
作者：
Soussan, P;Garreau, F;Kremsdorf, D
通讯作者：
Kremsdorf, D
影响因子：
14.9
作者：
Goujon M;McWilliam H;Li W;Valentin F;Squizzato S;Paern J;Lopez R
通讯作者：
Lopez R
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
3.7
作者：
Gunther, S;Sommer, G;Will, H
通讯作者：
Will, H