Dielectrophoresis: a review of applications for stem cell research.

Dielectrophoresis: a review of applications for stem cell research.
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DOI:
10.1155/2010/182581
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发表时间:
2010
影响因子:
--
通讯作者:
De Sousa P
De Sousa P
中科院分区:
其他
文献类型:
--
作者:
Pethig R;Menachery A;Pells S;De Sousa P

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介电泳法可以区分不同种群中不同的细胞身份,并监测与激活和克隆性扩张、凋亡和坏死相关的细胞状态变化,而不需要生化标记。已证明的能力包括从骨髓和外周血中浓缩造血干细胞,以及从脂肪组织中浓缩成人干细胞。最近的研究表明,这项技术可以在特定细胞表面蛋白出现之前预测神经干细胞分化后的最终命运。本文综述了细胞介电行为的特性,以及它们与干细胞研究和翻译应用的相关性。
Dielectrophoresis can discriminate distinct cellular identities in heterogeneous populations, and monitor cell state changes associated with activation and clonal expansion, apoptosis, and necrosis, without the need for biochemical labels. Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue. Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins. This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.
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