Lipolysis induced by leptin in rat adipose tissue from different anatomical locations
Lipolysis induced by leptin in rat adipose tissue from different anatomical locations
复制标题
瘦素诱导不同解剖部位大鼠脂肪组织的脂肪分解
DOI：
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发表时间：
2003
影响因子：
5
通讯作者：
M. Portillo
中科院分区：
文献类型：
--
作者：
V. Rodríguez;M. Macarulla;E. Echevarría;M. Portillo
摘要本工作的目的是比较大鼠体外条件下三种脂肪库（皮下、附睾和肾周）对瘦素的脂解反应。此外，评估年轻和成熟大鼠在这些组织对瘦素的反应方面的潜在差异。通过胶原酶消化分离6周龄和20周龄大鼠的脂肪细胞，并在不存在和存在瘦素（10−12-10−6 M）或异丙肾上腺素（10−6 M）的情况下进行体外孵育。通过孵育2小时内甘油释放到孵育培养基中来测量脂解。脂肪细胞对10 - 12 M至10 - 6 M的瘦素浓度有剂量依赖性反应。在所有组织中，诱导显著脂解作用的最低瘦素浓度为10−9 M。在两个年龄段的组织中，瘦素最大浓度（10 - 6 M）的影响没有显著差异。异丙肾上腺素（10−6 M）的脂解作用在成年大鼠的脂肪组织中显著降低;相反，在年轻大鼠和成年大鼠之间观察到瘦素（10−6 M）的作用没有显著差异。总之，大鼠脂肪组织对瘦素诱导的脂质动员的反应不存在解剖学特异性差异。此外，与年轻大鼠相比，成熟大鼠的瘦素作用并未降低。
Summary. The aim of the present work is to compare the lipolytic response of three fat depots (subcutaneous, epididymal and perirenal) to leptin under in vitro conditions in rats. Moreover an assessment of the potential differences between young and mature rats in terms of the response of these tissues to leptin is made. Adipocytes from 6- and 20-wk-old rats were isolated by collagenase digestion and incubated in vitro both in the absence and the presence of either leptin (10−12–10−6 M) or isoproterenol (10−6 M). Lipolysis was measured by the release of glycerol into the incubation medium over 2 hours of incubation. Adipocytes responded in a dose-dependent manner to leptin concentrations ranging from 10−12 M to 10−6 M. The lowest leptin concentration inducing a significant lipolytic effect was 10−9 M in all tissues. No significant differences in the effect of the maximal concentration of leptin (10−6 M) were observed among tissues for either age. The lipolytic effect of isoproterenol (10−6 M) was significantly reduced in adipose tissues from mature rats; in contrast no significant differences in the effect of leptin (10−6 M) were observed between young and mature rats. In summary, no anatomical-specific differences exist in the response of rat adipose tissue to lipid mobilization induced by leptin. Furthermore, this leptin action is not decreased in mature rats compared with young ones.
DOI：
10.1152/ajpendo.1998.275.2.e259
发表时间：
1998-08-01
影响因子：
5.1
作者：
Scarpace, PJ;Matheny, M
通讯作者：
Matheny, M
影响因子：
7.7
作者：
Scarpace, PJ;Matheny, M;Tümer, N
通讯作者：
Tümer, N
影响因子：
6.5
作者：
M. Lafontan;M. Berlan
通讯作者：
M. Lafontan;M. Berlan