Reversal of the relative expression of cardiac and skeletal alpha1 subunit isoforms of L-type calcium channel during in vitro myogenesis
Reversal of the relative expression of cardiac and skeletal alpha1 subunit isoforms of L-type calcium channel during in vitro myogenesis
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体外成肌过程中心脏和骨骼 L 型钙通道 α1 亚基异构体相对表达的逆转
DOI：
10.1007/s004240050291
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Guy Raymond
中科院分区：
文献类型：
--
作者：
L. Bulteau;Michel Cogné;C. Cognard;Guy Raymond
摘要心脏和骨骼肌的兴奋-收缩偶联（ECC）类型是不同的。 这些差异可以用ECC中分子实体的结构差异来解释，即肌膜的二氢吡啶（DHP）受体（L型钙通道的α1亚基）或肌浆网膜的兰尼碱受体。正如之前通过电生理学手段所证明的那样，在骨骼肌体外发育的第一阶段，两种类型的ECC共存，而在后期阶段，骨骼型ECC占主导地位。为了观察ECC的演变是否与α1亚基的表达有关，我们用北方印迹法测定了在体外肌形成过程中α1亚基的表达。对应于心脏亚型的mRNA存在于成肌细胞中（融合前），但膜片钳实验表明它们没有功能。融合后，骨骼和心脏mRNA在肌管中共表达，但强度不同：骨骼mRNA的表达（强度更高）在测试的后期阶段稳定，而心脏mRNA则下降。我们的结论是mRNA α1亚基亚型表达的演变可以部分解释体外肌发生过程中ECC特征的演变。
Abstract Cardiac and skeletal type of excitation-contraction coupling (ECC) are quite different. Those differences could be explained by structural ones in the molecular entities involved in ECC, ie dihydropyridines (DHP) receptors (α1 subunit of L-type calcium channels) of the sarcolemma or ryanodine receptors of the sarcoplasmic reticulum membrane. As previously demonstrated by means of electrophysiology, the two types of ECC coexist during the first stages of in vitro development of skeletal muscle, whereas the skeletal type predominates at the later ones. In order to see whether evolution of ECC could be correlated with the one of α1 subunit expression, we determined by Northern Blotting which isoforms of α1 subunit are expressed during the in vitro myogenesis. mRNA corresponding to the cardiac isoform are present in myoblasts (before fusion), but patch-clamp experiments showed that they are not functional. After fusion, skeletal and cardiac mRNA are coexpressed in myotubes, with different intensities: whereas expression of skeletal mRNA (which are the more intensive) stabilized at the later stages tested, cardiac mRNA decreased. We conclude that evolution in mRNA α1 subunit isoforms expression could partly explained evolution of ECC features during in vitro myogenesis.
心脏钙通道的 mRNA 在骨骼肌发育过程中表达。
影响因子：
2.7
作者：
Chaudhari,N;Beam,KG
通讯作者：
Beam,KG