Stem cell therapy for the bladder--where do we stand?
Stem cell therapy for the bladder--where do we stand?
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膀胱干细胞疗法——我们的处境如何？
DOI：
10.1016/j.juro.2010.12.014
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Lin,Ching-Shwun
中科院分区：
文献类型：
--
作者：
Lin,Ching-Shwun
Stem cell therapy for the bladder has thus far been conducted on an experimental basis in two areas: bladder dysfunction and tissue engineering. For bladder dysfunction, Nishijima et al 1 showed that intra-bladder injection of bone marrow cells (not sorted or tested for stem cell properties) restored bladder contraction in rats with underactive bladder due to bladder outlet obstruction (BOO). More recently, Huang et al 2 showed that intra-bladder or intravenous injection of adipose-derived stem cells (ADSCs) improved urodynamics and tissue parameters in a rat model of hyperlipidemia-associated overactive bladder (OAB). In the current issue of this Journal Woo et al 3 report that intravenous injection of bone marrow stem cells (BMSCs) in a mouse model of BOO resulted in the recruitment of the transplanted cells to the bladder with concomitant improvements in histological and functional parameters. Importantly, while Nishijima et al found evidence of smooth muscle differentiation from the transplanted cells, Huang et al and Woo et al found little or no such evidence.Stem cells’ therapeutic efficacy was originally thought to derive from their ability to differentiate into various cell types. In this regard several studies have shown that stem cells isolated from the embryo, bone marrow, amniotic fluid, and adipose tissue could differentiate into “bladder” smooth muscle cells. More recent studies have also shown that ADSCs and BMSCs could differentiate into the more complex urothelial cells 4, 5. However, it should be pointed out that evidence presented in most of these studies was based on detection of smooth muscle or urothelial markers in cultured stem cells that were maintained in certain conditioned media or co-cultured with native bladder cells. The few “in vivo” studies that presented evidence of smooth muscle differentiation in the bladder also need to be interpreted with caution. Perhaps the most provocative is a recent study on the reconstitution of neurogenic bladder dysfunction with skeletal muscle-derived stem cells (SkMSC)-several different cell types including Schwann cells, perineurial cells, and pericytes were suggested to have been differentiated from the transplanted cells 6. Further studies are needed to verify such claims.
DOI：
10.1016/j.juro.2009.11.012
发表时间：
2010-03
期刊：
The Journal of urology
影响因子：
--
作者：
Huang YC;Shindel AW;Ning H;Lin G;Harraz AM;Wang G;Garcia M;Lue TF;Lin CS
通讯作者：
Lin CS
影响因子：
4.5
作者：
Zhang, YY;Lin, HK;Kropp, BP
通讯作者：
Kropp, BP
影响因子：
14
作者：
Jack, Gregory S.;Zhang, Rong;Lee, Min;Xu, Yuhan;Wu, Ben M.;Rodriguez, Larissa V.
通讯作者：
Rodriguez, Larissa V.