Proteomic analysis of lipopolysaccharide-treated submandibular gland in rat.
Proteomic analysis of lipopolysaccharide-treated submandibular gland in rat.
复制标题
脂多糖处理的大鼠颌下腺的蛋白质组学分析。
DOI：
10.2209/tdcpublication.52.31
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
M. Kawaguchi
中科院分区：
文献类型：
--
作者：
K. Sawaki;T. Shinomiya;M. Okubo;E. Tsukagoshi;M. Ogane;Mototsugu Matsuura;M. Yoshikawa;M. Kawaguchi
我们研究了暴露于脂多糖（LPS）引起的炎症引起的下颌下腺（SMG）蛋白质谱的变化，目的是识别受损腺体的潜在分子标记。通过逆行导管注射将脂多糖（2.5μg）直接单侧给予大鼠SMG。治疗后12小时，切除腺体并通过二维差异凝胶电泳和基质辅助激光解吸/电离飞行时间质谱法鉴定蛋白质。与对侧腺体相比，LPS 处理腺体中的许多蛋白质显示出显着变化。特别值得注意的是泛素（一种高度保守的小调节蛋白）和钙颗粒蛋白 B（在炎症中具有免疫功能）的增加。与细胞凋亡和应激相关的蛋白质也在发炎的腺体中表现出变化。这项研究的结果表明，蛋白质修饰的泛素系统参与了 LPS 诱导的唾液腺炎症，并且许多特定蛋白质可能可用作监测发炎或受损腺体的分子标记。
We investigated changes in the protein profile of submandibular gland (SMG) with inflammation induced by exposure to lipopolysaccharide (LPS) with the aim of identifying potential molecular markers of injured gland. Lipopolysaccharide (2.5µg) was directly administered into rat SMG unilaterally by retrograde ductal injection. At 12hr after treatment, the gland was excised and the proteins identified by two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Many proteins in the LPS-treated gland showed a marked change compared to those in the contralateral gland. Of particular note were increases in ubiquitin, a highly-conserved small regulatory protein and in calgranulin B, which has an immunological function in inflammation. Proteins related to apoptosis and stress also showed change in the inflamed gland. The results of this study suggest that the ubiquitin system of protein modification is involved in LPS-induced inflammation in salivary gland, and that a number of specific proteins might be applicable as molecular markers in the monitoring of inflamed or injured gland.
影响因子：
4.4
作者：
Saraswathy S;Rao NA
通讯作者：
Rao NA
影响因子：
8.6
作者：
Yamano, S;Atkinson, JC;Fox, PC
通讯作者：
Fox, PC