Identification of S-genotypes and novel S-RNase alleles in Prunus mume

Identification of S-genotypes and novel S-RNase alleles in Prunus mume
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梅S基因型和新S-RNase等位基因的鉴定

DOI:
10.1080/14620316.2008.11512445
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发表时间:
2008-01
影响因子:
1.9
通讯作者:
Zhang, S. L.
Zhang, S. L.
中科院分区:
农林科学4区
文献类型:
--
作者:
Chen, Q. X.;Wu, J.;Heng, W.;Huang, S. X.;Wu, H. Q.;Zhang, S. L.

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日本杏品种是自交不亲和的,果园需要授粉品种来保证最佳的果实产量。因此,鉴定乌梅品种的自交不亲和基因型对选择传粉媒介和育种是有帮助的。利用引物扩增S-RNase基因可变第二内含子,对11个中国品种和1个引种品种的s -基因型进行了分析。s基因分型结果如下:‘丹桃粉’为S4S12;S3S4为“Duoezhusha”;S12S13为“补板调质”;S15S16为“Hongding”;S13S14为“龙烟梅”;S5S11代表“武藏野”;S10S14为“消暑竹肝”;“清风”、“青佳”、“西叶青”、“大叶青”和“长农17”的S3S15。我们鉴定出7个新的S-RNase等位基因,分别为S10-RNase至S16-RNase,储存在GenBank中,登录号分别为:DQ011150、DQ201191、DQ201192、DQ345781、DQ768219、DQ903312和EF990750。
Summary Prunus mume (Japanese apricot) cultivars are self-incompatible and orchards require pollinator cultivars to guarantee optimal fruit production. Identification of the self-incompatibility (S) genotypes of P. mume cultivars is therefore useful for choosing pollinators and for breeding. To elucidate the S-genotypes of eleven Chinese and one introduced cultivar, PCR was performed with primers amplifying the variable second intron of the S-RNase gene. The S-genotyping results were as follows: S4S12 for ‘Dantaofen’; S3S4 for ‘Duoezhusha’; S12S13 for ‘Fubantiaozhi’; S15S16 for ‘Hongding’; S13S14 for ‘Longyanmei’; S5S11 for ‘Musashino’; S10S14 for ‘Xiaoyezhugan’; and S3S15 for ‘Qingfeng’,‘Qingjia’, ‘Xiyeqing’, ‘Dayeqing’ and ‘Changnong 17’. We identified seven new S-RNase alleles, S10-RNase to S16-RNase, deposited in GenBank under the accession numbers: DQ011150, DQ201191, DQ201192, DQ345781, DQ768219, DQ903312 and EF990750, respectively.
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发表时间: 2004-08
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