FLOWERING LOCUS T2 regulates spike development and fertility in temperate cereals.
FLOWERING LOCUS T2 regulates spike development and fertility in temperate cereals.
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DOI：
10.1093/jxb/ery350
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发表时间：
2019-01-01
影响因子：
6.9
通讯作者：
Dubcovsky J
中科院分区：
文献类型：
--
作者：
Shaw LM;Lyu B;Turner R;Li C;Chen F;Han X;Fu D;Dubcovsky J
开花基因T2(FT2)在发育中的小麦穗的远端表达，对每穗小穗数起调控作用。开花基因T2(FT2)是温带牧草中与FT1开花基因距离最近的一对基因。在这里，我们发现FT2的过度表达导致早熟开花和小穗数量减少，而RNA干扰下调导致开花延迟和小花充实率降低。同样，四倍体小麦中FT2同源基因的截断突变会延迟开花(2-4d)并降低育性。小麦ft2突变体的每穗小穗数也显著增加，穗发育期较长可能是导致抽穗期延迟的原因之一。在小麦叶片中，FT2的表达晚于FT1，这表明FT2在生殖启动过程中的作用相对较小。FT2转录本在顶端分生组织中检测到，并在幼穗发育过程中增加。发育穗的横切面显示，在形成新小穗的远端部分，FT2的转录水平最高。我们的结果表明，在小麦中，FT2在穗部发育和育性中起重要作用，但在营养枝顶端分生组织和生殖枝顶端分生组织之间的时间转换中作用有限。
FLOWERING LOCUS T2 (FT2) is expressed in the distal part of the developing wheat spike and contributes to the regulation of the number of spikelets per spike. FLOWERING LOCUS T2 (FT2) is the closest paralog of the FT1 flowering gene in the temperate grasses. Here we show that overexpression of FT2 in Brachypodium distachyon and barley results in precocious flowering and reduced spikelet number, while down-regulation by RNA interference results in delayed flowering and a reduced percentage of filled florets. Similarly, truncation mutations of FT2 homeologs in tetraploid wheat delayed flowering (2–4 d) and reduced fertility. The wheat ft2 mutants also showed a significant increase in the number of spikelets per spike, with a longer spike development period potentially contributing to the delayed heading time. In the wheat leaves, FT2 was expressed later than FT1, suggesting a relatively smaller role for FT2 in the initiation of the reproductive phase. FT2 transcripts were detected in the shoot apical meristem and increased during early spike development. Transversal sections of the developing spike showed the highest FT2 transcript levels in the distal part, where new spikelets are formed. Our results suggest that, in wheat, FT2 plays an important role in spike development and fertility and a limited role in the timing of the transition between the vegetative and reproductive shoot apical meristem.
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影响因子：
7.4
作者：
Michaels, SD;Himelblau, E;Amasino, RM
通讯作者：
Amasino, RM
影响因子：
3.3
作者：
Faure, Sebastien;Higgins, Janet;Laurie, David A.
通讯作者：
Laurie, David A.
影响因子：
9.2
作者：
Jaeger, Katja E.;Wigge, Philip A.
通讯作者：
Wigge, Philip A.
影响因子：
7.2
作者：
Shaw, Lindsay M.;Turner, Adrian S.;Laurie, David A.
通讯作者：
Laurie, David A.
影响因子：
56.9
作者：
Wigge, PA;Kim, MC;Weigel, D
通讯作者：
Weigel, D