Biological characterization of a novel in vitro cell irradiator.
Biological characterization of a novel in vitro cell irradiator.
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DOI：
10.1371/journal.pone.0189494
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kimple RJ
中科院分区：
文献类型：
--
作者：
Fowler TL;Fisher MM;Bailey AM;Bednarz BP;Kimple RJ
To evaluate the overall robustness of a novel cellular irradiator we performed a series of well-characterized, dose-responsive assays to assess the consequences of DNA damage. We used a previously described novel irradiation system and a traditional 137Cs source to irradiate a cell line. The generation of reactive oxygen species was assessed using chloromethyl-H2DCFDA dye, the induction of DNA DSBs was observed using the comet assay, and the initiation of DNA break repair was assessed through γH2AX image cytometry. A high correlation between physical absorbed dose and biologic dose was seen for the production of intracellular reactive oxygen species, physical DNA double strand breaks, and modulation of the cellular double stand break pathway. The results compared favorably to irradiation with a traditional 137Cs source. The rapid, straightforward tests described form a reasonable approach for biologic characterization of novel irradiators. These additional testing metrics go beyond standard physics testing such as Monte Carlo simulation and thermo-luminescent dosimeter evaluation to confirm that a novel irradiator can produce the desired dose effects in vitro. Further, assessment of these biological metrics confirms that the physical handling of the cells during the irradiation process results in biologic effects that scale appropriately with dose.
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影响因子：
11.2
作者：
Kimple RJ;Smith MA;Blitzer GC;Torres AD;Martin JA;Yang RZ;Peet CR;Lorenz LD;Nickel KP;Klingelhutz AJ;Lambert PF;Harari PM
通讯作者：
Harari PM
影响因子：
4.3
作者：
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通讯作者：
Williams, Terence M.
影响因子：
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作者：
Bordelon, David E.;Zhang, Jian;Chang, Sha
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Chang, Sha
影响因子：
3.8
作者：
McCaw, Travis J.;Micka, John A.;DeWerd, Larry A.
通讯作者：
DeWerd, Larry A.
影响因子：
3.4
作者：
Wan, XS;Zhou, ZZ;Kennedy, AR
通讯作者：
Kennedy, AR