Effects of early life seizures on coordination of hippocampal-prefrontal networks: Influence of sex and dynamic brain states.
Effects of early life seizures on coordination of hippocampal-prefrontal networks: Influence of sex and dynamic brain states.
复制标题
早期癫痫发作对海马前额外网络协调的影响：性别和动态大脑状态的影响。
作者：
Niedecker RW;Kloc ML;Holmes GL;Barry JM
生命早期癫痫（ELS）改变了学习和记忆基础上神经元回路的活动依赖性成熟。癫痫引起的认知障碍的病理生理机制尚不完全清楚，性别和动态大脑状态等与认知结果有关的关键变量尚未得到探索。我们假设，与对照（CTL）大鼠相比，ELS大鼠会表现出与海马和内侧前额叶皮层（mPFC）之间动态神经信号协调受损相关的空间认知缺陷。从出生后第15天开始，雄性和雌性大鼠幼仔在10天内给予50次氟甲醚诱发癫痫发作。作为成年人，空间认知是通过在旋转舞台上主动回避来测试的。微丝四极电极植入mPFC和CA1子场。在主动回避和睡眠时，记录并分析每个区域的单细胞和局部场。ELS雄性大鼠表现出回避障碍，而雌性大鼠未受影响。在回避过程中，CTL雌性海马- mpfc的一致性高于CTL雄性。与CTL男性相比，ELS男性学习者在theta带宽内表现出更高的一致性，并且mPFC细胞活动的爆发时间发生了改变。海马体- mpfc一致性水平可预测主动回避空间任务的认知结果。ELS后的空间认知结果与性别有关，女性表现优于男性。学习任务的ELS男性在低θ波频率表现出增加的mPFC相干水平，这可能弥补了ELS对mPFC细胞计时的影响。这些结果表明，连贯性可以作为ELS后空间认知结果的生物标志物，并强调分析性别和动态认知作为变量在理解癫痫对发育中的大脑的影响方面的重要性。
Early-life seizures (ELS) alter activity-dependent maturation of neuronal circuits underlying learning and memory. The pathophysiological mechanisms underpinning seizure-induced cognitive impairment are not fully understood and critical variables such as sex and dynamic brain states with regard to cognitive outcomes have not been explored. We hypothesized that in comparison to control (CTL) rats, ELS rats would exhibit deficits in spatial cognition correlating with impaired dynamic neural signal coordination between the hippocampus and medial prefrontal cortex (mPFC). Male and female rat pups were given 50 flurothyl-induced seizures over 10 days starting at postnatal day (P) 15. As adults, spatial cognition was tested through active avoidance on a rotating arena. Microwire tetrodes were implanted in the mPFC and CA1 subfield. Single cells and local field were recorded and analyzed in each region during active avoidance and sleep. ELS males exhibited avoidance impairments while female rats were unaffected. During avoidance, hippocampus-mPFC coherence was higher in CTL females than CTL males across bandwidths. In comparison to CTL males, ELS male learners exhibit increased coherence within theta bandwidth as well as altered burst-timing in mPFC cell activity. Hippocampus-mPFC coherence levels are predictive of cognitive outcome in the active avoidance spatial task. Spatial cognitive outcome post ELS is sex-dependent as females fare better than males. ELS males that learn the task exhibit increased mPFC coherence levels at low-theta frequency which may compensate for ELS effects on mPFC cell timing. These results suggest that coherence may serve as a biomarker for spatial cognitive outcome post ELS and emphasize the significance of analyzing sex and dynamic cognition as variables in understanding seizure effects on the developing brain.
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影响因子：
3.5
作者：
Blanquat, Paul De Saint;Hok, Vincent;Chaillan, Franck A.
通讯作者：
Chaillan, Franck A.
影响因子：
5.3
作者：
Cancedda, Laura;Fiumelli, Hubert;Poo, Mu-ming
通讯作者：
Poo, Mu-ming
影响因子：
2.9
作者：
Hasselmo, ME;Bodelón, C;Wyble, BP
通讯作者：
Wyble, BP
影响因子：
5.5
作者：
Akman, Ozlem;Moshe, Solomon L.;Galanopoulou, Aristea S.
通讯作者：
Galanopoulou, Aristea S.
DOI：
10.1126/science.aan8869
发表时间：
2017-10-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Buzsáki G;Llinás R
通讯作者：
Llinás R