PAX6 dosage effects on corneal development, growth, and wound healing.
PAX6 dosage effects on corneal development, growth, and wound healing.
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PAX6剂量对角膜发育，生长和伤口愈合的影响。
DOI：
10.1002/dvdy.21528
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发表时间：
2008-05
影响因子：
2.5
通讯作者：
Collinson, J. Martin
中科院分区：
文献类型：
--
作者：
Dora, Natalie;Ou, Jingxing;Kucerova, Romana;Parisi, Ida;West, John D.;Collinson, J. Martin
使用Pax 6过表达（PAX 77）转基因小鼠研究角膜生长和发育过程中正确剂量的转录因子Pax 6的需求。由于出生后生长失败，转基因动物具有小角膜表型，与角膜上皮细胞层数量减少有关。使用溴脱氧尿苷、p63、细胞周期蛋白E和磷酸化组蛋白-3标记监测细胞周期进展：PAX 77+中的增殖率高于野生型，而不伴随凋亡的增加。因此，增殖失败并不构成小角膜的基础。PAX 77+角膜上皮细胞的细胞角蛋白-12水平降低，并表现出严重的伤口愈合延迟，与Pax 6 +/-小鼠相反，不能通过外源性生长因子调节。PAX 77+晶状体显示透镜纤维分化部分失效。数据表明，前眼发育对Pax 6剂量非常敏感。尽管Pax 6杂合子和过表达小鼠的眼睛表型之间存在相似性，但也存在显著差异。
The requirement for correct dosage of the transcription factor Pax6 during corneal growth and development was investigated using the Pax6-overexpressing (PAX77) transgenic mouse. Transgenics had a microcornea phenotype due to failure of postnatal growth, associated with reduction in the number of cells layers in the corneal epithelium. Cell cycle progression was monitored using bromodeoxyuridine, p63, cyclin E, and phosphohistone-3 labeling: proliferation rates were higher in PAX77+ than wild-type, without a concomitant increase in apoptosis. Hence, failure of proliferation did not underlie microcornea. PAX77+ corneal epithelia had reduced levels of cytokeratin-12, and exhibited severe wound healing delay that, in contrast to Pax6+/- mice, could not be modulated by exogenous growth factors. PAX77+ lenses showed partial failure of lens fiber differentiation. The data demonstrate that anterior eye development is very sensitive to Pax6 dosage. Although there are similarities between the eye phenotype of Pax6 heterozygotes and overexpressing mice, there are also striking differences.
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影响因子：
64.8
作者：
HILL, RE;FAVOR, J;VANHEYNINGEN, V
通讯作者：
VANHEYNINGEN, V
DOI：
10.1073/pnas.161144098
发表时间：
2001-08-14
影响因子：
11.1
作者：
Collinson, JM;Quinn, JC;Hill, RE
通讯作者：
Hill, RE
影响因子：
13.7
作者：
Holland, EJ;Djalilian, AR;Schwartz, GS
通讯作者：
Schwartz, GS
影响因子：
3.4
作者：
Liu, JJ;Kao, WWY;Wilson, SE
通讯作者：
Wilson, SE
影响因子：
4.4
作者：
Kucerova, Romana;Ou, Jingxing;Collinson, J. Martin
通讯作者：
Collinson, J. Martin