Blood metabolic and physiological profiles of Bama miniature pigs at different growth stages.
Blood metabolic and physiological profiles of Bama miniature pigs at different growth stages.
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巴马小型猪不同生长阶段的血液代谢和生理特征
DOI：
10.1186/s40813-022-00278-7
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发表时间：
2022-08-08
影响因子：
3.4
通讯作者：
Liang J
中科院分区：
文献类型：
--
作者：
Mo J;Lu Y;Xing T;Xu D;Zhang K;Zhang S;Wang Y;Yan G;Lan G;Liang J
年龄在六 (6 M) 至十二个月 (12 M) 之间的巴马小型猪通常作为实验猪用于人类医学研究。然而，6 月龄至 12 月龄猪的血清代谢谱差异仍不清楚。本研究旨在确定血液中存在的代谢和生理特征，以进一步解释巴马小型猪生长的变化。我们采集了 6 月龄、8 月龄（8 月龄）、10 月龄（10 月龄）和 12 月龄健康广西巴马小型猪的血液样本。总共测量了 20 项血液生理指数 (BPI)：白细胞指数 7 项、红细胞指数 8 项、血小板指数 5 项。基于液相色谱和质谱的非靶向代谢组学方法用于分析代谢物的差异。使用 Spearman 相关性和 Benjamini-Hochberg 调整计算差异之间的关联。选择 100 种最丰富的差异代谢物来分析其代谢谱。不同年龄BPI无显着差异，但中细胞比例和红细胞数随年龄增加而增加。巴马小型猪的 7 个 BPI 更接近人类 BPI，而不是小鼠 BPI。在 6 M 与 12 M 和 8 M 与 12 M 中分别鉴定出总共 14 种和 25 种显着差异代谢物。总的来说，6 月龄和 8 月龄猪中的 9 种和 18 种氨基酸及其衍生物的浓度显着低于 12 月龄猪。它们被确定为 6 M 与 12 M 年龄组和 8 M 与 12 M 年龄组之间显着不同的核心代谢物。一半的富集途径是氨基酸代谢途径。六种氨基酸（dl-色氨酸、苯乙酰甘氨酸、胞壁酸、N-乙酰鸟氨酸、l(−)-哌啶酸和肌酸）及其衍生物的浓度随着年龄的增长而增加。前 100 种最丰富的代谢物中总共有 61 种被注释。代谢谱包含 14 种氨基酸和衍生物、6 种胆汁酸和衍生物、19 种脂肪酸和衍生物以及其他 22 种。发现脂肪酸和衍生物的浓度与氨基酸和衍生物的浓度成反比。这些发现表明，12 月龄猪的 MID 细胞比率、红细胞计数和氨基酸水平较高，可以作为巴马小型猪身体功能随时间改善的指标，与人类发育过程中的情况类似。这使得猪成为比小鼠更合适的医学模型生物。本研究结果仅限于巴马小型猪近交系血液代谢特征，在研究其他猪群时应考虑品种、年龄、性别、健康状况和营养水平等影响因素的影响。在线版本包含可在 10.1186/s40813-022-00278-7 获取的补充材料。
Bama miniature pigs aged between six (6 M) and twelve months (12 M) are usually used in human medical research as laboratory pigs. However, the difference in serum metabolic profiles from 6 to 12 M-old pigs remains unclear. This study aimed to identify the metabolic and physiological profiles present in the blood to further explain changes in Bama miniature pig growth. We collected blood samples from 6 M-, eight-month- (8 M-), ten-month- (10 M-), and 12 M-old healthy Guangxi Bama miniature pigs. A total of 20 blood physiological indices (BPIs) were measured: seven for white blood cells, eight for red blood cells, and five for platelet indices. Liquid chromatography and mass spectrometry-based non-targeted metabolomic approaches were used to analyze the difference in metabolites. The associations between the differences were calculated using Spearman correlations with Benjamini–Hochberg adjustment. The 100 most abundant differential metabolites were selected for analysis of their metabolic profiles. There were no significant differences in BPIs at different ages, but the mid cell ratio and red blood cell number increased with age. Seven BPIs in Bama miniature pigs were closer to human BPIs than to mouse BPIs. A total of 14 and 25 significant differential metabolites were identified in 6 M vs. 12 M and 8 M vs. 12 M, respectively. In total, 9 and 18 amino acids and their derivatives showed significantly lower concentrations in 6 M- and 8 M-old pigs than in 12 M-old pigs. They were identified as the core significantly different metabolites between the age groups 6 M vs. 12 M and 8 M vs. 12 M. Half of the enriched pathways were the amino acids metabolism pathways. The concentration of six amino acids (dl-tryptophan, phenylacetylglycine, muramic acid, N-acetylornithine, l(−)-pipecolinic acid, and creatine) and their derivatives increased with age. A total of 61 of the top 100 most abundant metabolites were annotated. The metabolic profiles contained 14 amino acids and derivatives, six bile acids and derivatives, 19 fatty acids and derivatives, and 22 others. The concentrations of fatty acids and derivatives were found to be inversely proportional to those of amino acids and derivatives. These findings suggest high levels of MID cell ratio, red blood count, and amino acids in 12 M-old pigs as indicators for improved body function over time in Bama miniature pigs, similar to those in human development. This makes the pig a more suitable medical model organism than the mouse. The results of this study are limited to the characteristics of blood metabolism in the inbred Bama miniature pigs, and the effects of impacting factors such as breed, age, sex, health status and nutritional level should be considered when studying other pig populations. The online version contains supplementary material available at 10.1186/s40813-022-00278-7.
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影响因子：
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作者：
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影响因子：
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影响因子：
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DOI：
10.1016/j.jchromb.2020.122455
发表时间：
2021-01-01
影响因子：
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作者：
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通讯作者：
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