An injectable thiol-acrylate poly(ethylene glycol) hydrogel for sustained release of methylprednisolone sodium succinate.
An injectable thiol-acrylate poly(ethylene glycol) hydrogel for sustained release of methylprednisolone sodium succinate.
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DOI：
10.1016/j.biomaterials.2010.08.106
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Langer, Robert
中科院分区：
文献类型：
--
作者：
Pritchard, Christopher D.;O'Shea, Timothy M.;Siegwart, Daniel J.;Calo, Eliezer;Anderson, Daniel G.;Reynolds, Francis M.;Thomas, John A.;Slotkin, Jonathan R.;Woodard, Eric J.;Langer, Robert
临床可用的可注射水凝胶面临与注射后溶胀和来自未反应成分的毒性相关的技术挑战，这阻碍了其作为外科生物材料的性能。为了克服这些挑战，我们开发了一种系统，其中通过在水性介质中硫醇和丙烯酸酯之间的共轭迈克尔加成来控制化学凝胶化，具有97%的单体转化率和6 wt.%溶胶分数。水凝胶在平衡时表现出脱水收缩，减少至其初始体积的59.7%。它具有类似于人体软组织的机械性能，弹性模量为189.8 kPa。此外，6.9 nm的筛孔尺寸导致甲泼尼龙琥珀酸钠的持续释放，负载效率为2 mg/mL。用50 μg/mL的寡赖氨酸肽官能化导致新鲜分离的鼠间充质干细胞的附着。水凝胶的物理、化学和生物学性质的合理设计使其成为可注射应用的潜在有希望的候选者。
Clinically available injectable hydrogels face technical challenges associated with swelling after injection and toxicity from unreacted constituents that impede their performance as surgical biomaterials. To overcome these challenges, we developed a system where chemical gelation was controlled by a conjugate Michael addition between thiol and acrylate in aqueous media, with 97% monomer conversion and 6 wt.% sol fraction. The hydrogel exhibited syneresis on equilibration, reducing to 59.7% of its initial volume. It had mechanical properties similar to soft human tissue with an elastic modulus of 189.8 kPa. Furthermore, a mesh size of 6.9 nm resulted in sustained release of methylprednisolone sodium succinate with a loading efficiency of 2 mg/mL. Functionalization with 50 µg/mL of an oligolysine peptide resulted in attachment of freshly isolated murine mesenchymal stem cells. The rational design of the physical, chemical and biological properties of the hydrogel makes it a potentially promising candidate for injectable applications.
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