Effect of different derivatization protocols on the calculation of trophic position using amino acids compound-specific stable isotopes
Effect of different derivatization protocols on the calculation of trophic position using amino acids compound-specific stable isotopes
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不同衍生化方案对使用氨基酸化合物特异性稳定同位素计算营养位置的影响
DOI：
10.3389/fmars.2020.561568
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
D. Tchernov
中科院分区：
文献类型：
--
作者：
Stephane Martinez;Maya Lalzer;E. Shemesh;S. Einbinder;Beverly Goodman Tchernov;D. Tchernov
氨基酸化合物特异性氮稳定同位素（AA-CSIA）是生态学研究营养系统动力学的新兴工具。虽然它已经成功地用于一系列环境和研究地点的几项独立研究，但研究人员在确定营养位置值时遇到了计算问题。大多数研究引入了对营养位置方程计算常数的修改，但在比较单独研究结果时未能考虑方程的变化。这种方法的接受与潜在的假设有关，即在不同的方法中不会发生外源氮原子的添加，因此，这种变化不应该影响结果。在本文中，我们评估了EZfaast氨基酸衍生化试剂盒（氯甲酸酯）的使用，并将其与其他两种衍生化方法的同位素结果进行了比较。我们强调了与AA-CSIA合作的新考虑因素，这可能解释了结果中的一些变化，并引导研究人员修改方程中的常数。这可能需要开发每个衍生化方法的唯一常数，以便能够比较不同研究中的营养位置结果。
Amino acids compound-specific nitrogen stable isotope (AA-CSIA) is an emerging tool in ecology for understanding trophic system dynamics. While it has been successfully used for several independent studies across a range of environments and study locations, researchers have encountered calculation issues for determining trophic position values. Most studies introduce modifications to the constants of trophic position equation calculations, but then fail to account for the equation variations when comparing the results of separate research studies. The acceptance of this approach is related to the underlying presumption that no addition of the exogenous nitrogen atom occurs in the different methods and, therefore, such variations should not affect the outcome. In this paper, we evaluate the use of the EZfaast amino acid derivatization kit (chloroformate) and compare it to the isotopic results of two other derivatization methods. We highlight new considerations for working with AA-CSIA that might account for some of the variations in the results and lead researchers to modify constants in the equation. This likely requires developing the unique constants per derivatization method in order to be able to compare the trophic position results across different studies.
影响因子：
2.7
作者：
Nielsen, Jens M.;Popp, Brian N.;Winder, Monika
通讯作者：
Winder, Monika