Endogenous activation of glycine and NMDA receptors in lamprey spinal cord during fictive locomotion
Endogenous activation of glycine and NMDA receptors in lamprey spinal cord during fictive locomotion
复制标题
虚构运动过程中七鳃鳗脊髓中甘氨酸和 NMDA 受体的内源性激活
作者：
S. Alford;T. Williams
士的宁可以消除由感觉刺激引起的虚构运动中的左右交替。在所有剂量(0.5-20微米)的士的宁存在的情况下，可以看到强健的节律活动，特征是每个节段水平的左右共同激活。腹根突起和吻尾协调所占周期的比例与没有士的宁的情况相似。此外，顺式-2，3-哌啶二酸(PDA)、2-氨基-5-磷酸戊酸(APV)或从灌流液中去除镁离子(如在没有士的宁的情况下)可取消这种节律。在马钱子碱存在的刺激过程中，对腹角神经元施加电压钳，其保持电位与腹根爆发相关的平台电位负相关，而与爆发间期的电位正相关。在前根爆发时可见内向电流，而在爆发终止或间歇爆发时则未见外向电流。结果表明，在内源性释放NMDA受体激动剂诱导的虚拟运动中，左右交替依赖于甘氨酸能传递。此外，有证据表明，在没有甘氨酸能传递的情况下，猝发终止可能依赖于NMDA受体相关的电压敏感过程。
Strychnine is shown to abolish left-right alternation in fictive locomotion induced by sensory stimulation. Robust rhythmic activity, characterized by left-right coactivation at each segmental level, is seen in the presence of strychnine at all doses used (0.5–20 microM). The proportion of the cycle occupied by the ventral root bursts and the rostral-caudal coordination is similar to that seen in the absence of strychnine. Furthermore, the rhythm is abolished by cis-2,3-piperidine dicarboxylic acid (PDA), 2-amino-5-phosphonovalerate (APV), or the removal of Mg2+ from the perfusate, as in the absence of strychnine. Voltage clamp was applied to ventral horn neurons during stimulation in the presence of strychnine, with holding potentials negative to the plateau potential associated with a ventral root burst but positive to the potential in the interburst. Inward current was seen during the ventral root burst, but no outward current was seen at burst termination or during the interburst. The results indicate that in fictive locomotion induced by endogenous release of NMDA receptor agonists, left-right alternation is dependent on glycinergic transmission. Furthermore, evidence is provided that in the absence of glycinergic transmission, burst termination may depend on NMDA receptor- linked voltage-sensitive processes.
鉴定了七鳃鳗脊髓中的神经元及其在虚构游泳中的作用。
DOI：
--
发表时间：
1983
期刊：
Symposia of the Society for Experimental Biology
影响因子：
--
作者：
Rovainen,CM
通讯作者：
Rovainen,CM
兴奋性神经传递激活七鳃鳗脊髓运动系统神经元的电压依赖性特性。
影响因子：
2.5
作者：
Alford,S;Sigvardt,KA
通讯作者：
Sigvardt,KA
影响因子：
56.9
作者：
BUCHANAN, JT;GRILLNER, S
通讯作者：
GRILLNER, S