The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line.
The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line.
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DOI：
10.1016/j.neuro.2014.12.010
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发表时间：
2015-01
期刊：
影响因子：
3.4
通讯作者：
Coffin AB
中科院分区：
文献类型：
--
作者：
Hayashi L;Sheth M;Young A;Kruger M;Wayman GA;Coffin AB
双酚A（BPA）和多氯联苯（PCB）等环境毒物在我们的供水，土壤和许多食品中普遍存在，并会深刻影响中枢神经系统。BPA和PCB都可以扰乱内分泌信号，这对听觉发育和功能很重要，但这些毒物对听觉外周的影响尚不清楚。在这项研究中，我们研究了PCB-95和BPA对斑马鱼仔鱼侧线发育，功能和再生的影响。侧线是鱼类外部的机械感觉毛细胞系统，与哺乳动物内耳中的毛细胞同源。我们发现PCB-95对侧线发育或毛细胞存活没有影响。BPA也不影响侧线发育，但对毛细胞的存活和再生有显着影响。BPA诱导的毛细胞损失具有剂量和时间依赖性，1 μM或更高浓度的BPA在24小时暴露期间杀死侧线毛细胞。药理学操作实验表明，BPA通过激活氧化应激途径杀死毛细胞，类似于之前报道的BPA在其他组织中的毒性。我们还观察到，被新霉素（一种已知的耳毒素）杀死的毛细胞在BPA存在时无法正常再生，这表明水生环境中的BPA可能会阻碍鱼类的先天再生反应。总的来说，这些数据表明，BPA可能对水生生物和陆生生物（包括人类）的感觉系统产生有害影响。
Environmental toxicants such as bisphenol-A (BPA) and polychlorinated biphenyls (PCBs) are prevalent in our water supply, soil, and many food products and can profoundly affect the central nervous system. Both BPA and PCBs can disrupt endocrine signaling, which is important for auditory development and function, but the effect of these toxicants on the auditory periphery is not understood. In this study we investigated the effect of PCB-95 and BPA on lateral line development, function, and regeneration in larval zebrafish. The lateral line is a system of mechanosensory hair cells on the exterior of the fish that are homologous to the hair cells located in the mammalian inner ear. We found that PCB-95 had no effect on lateral line development or hair cell survival. BPA also did not affect lateral line development, but instead had a significant effect on both hair cell survival and regeneration. BPA-induced hair cell loss is both dose-and time-dependent, with concentrations of 1 μM or higher killing lateral line hair cells during a 24 hr exposure period. Pharmacologic manipulation experiments suggest that BPA kills hair cells via activation of oxidative stress pathways, similar to prior reports of BPA toxicity in other tissues. We also observed that hair cells killed with neomycin, a known ototoxin, failed to regenerate normally when BPA was present, suggesting that BPA in aquatic environments could impede innate regenerative responses in fishes. Collectively, these data demonstrate that BPA can have detrimental effects on sensory systems, both in aquatic life and perhaps in terrestrial organisms, including humans.
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