Comparative analysis of protein-protein interactions in the defense response of rice and wheat.
Comparative analysis of protein-protein interactions in the defense response of rice and wheat.
复制标题
蛋白质 - 蛋白质相互作用在大米和小麦的防御反应中的比较分析。
DOI：
10.1186/1471-2164-14-166
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发表时间：
2013-03-12
期刊：
影响因子：
4.4
通讯作者：
Dubcovsky J
中科院分区：
文献类型：
--
作者：
Cantu D;Yang B;Ruan R;Li K;Menzo V;Fu D;Chern M;Ronald PC;Dubcovsky J
尽管小麦作为主要的主食作物的重要性以及病害对其全球生产的负面影响，但小麦抗性反应中涉及的遗传机制和基因相互作用仍然知之甚少。水稻基因组的完整序列为小麦的遗传和基因组学研究提供了一个非常有用的平行路线图。最近在水稻中构建的防御反应相互作用组有可能进一步增强水稻的进展向小麦和其他禾本科植物的转化。本研究的目的是确定在水稻和小麦防御反应相互作用组中蛋白质-蛋白质相互作用的保守程度。作为切入点，我们选择了作为水稻防御反应关键调节因子的蛋白质：RAR 1/SGT 1/HSP 90蛋白复合物，NPR 1，XA 21和XB 12（XA 21相互作用蛋白12）。利用现有的小麦序列数据库和系统发育分析，我们确定并克隆了这四种水稻蛋白质的小麦直向同源物，包括最近重复的旁系同源物，以及它们已知的直接相互作用物，并使用酵母双杂交（Y2 H）试验测试了86种二元蛋白质相互作用。小麦蛋白质之间的所有相互作用进一步测试使用在植物双分子荧光互补（BiFC）。83%的已知水稻相互作用被证实时，小麦蛋白质与水稻互作剂和76%的小麦蛋白质对被证实。对于所鉴定的直系同源小麦蛋白，RAR 1/SGT 1/HSP 90、NPR 1和XB 12节点中的所有相互作用都得到了证实，而在以XA 21为中心的相互作用组节点中，只有44%的相互作用得到了证实。我们推测，这种减少可能与不同的亚功能化历史的多个重复发生在这个基因家族后，小麦和水稻谱系的分歧。作为水稻防御反应的关键调节因子的蛋白质之间的相互作用的观察到的高度保守性表明，现有的水稻相互作用组可用于预测小麦中的相互作用。对于在两个谱系中经历了不同的复制和子功能化历史的节点，这样的预测不太可靠。
Despite the importance of wheat as a major staple crop and the negative impact of diseases on its production worldwide, the genetic mechanisms and gene interactions involved in the resistance response in wheat are still poorly understood. The complete sequence of the rice genome has provided an extremely useful parallel road map for genetic and genomics studies in wheat. The recent construction of a defense response interactome in rice has the potential to further enhance the translation of advances in rice to wheat and other grasses. The objective of this study was to determine the degree of conservation in the protein-protein interactions in the rice and wheat defense response interactomes. As entry points we selected proteins that serve as key regulators of the rice defense response: the RAR1/SGT1/HSP90 protein complex, NPR1, XA21, and XB12 (XA21 interacting protein 12). Using available wheat sequence databases and phylogenetic analyses we identified and cloned the wheat orthologs of these four rice proteins, including recently duplicated paralogs, and their known direct interactors and tested 86 binary protein interactions using yeast-two-hybrid (Y2H) assays. All interactions between wheat proteins were further tested using in planta bimolecular fluorescence complementation (BiFC). Eighty three percent of the known rice interactions were confirmed when wheat proteins were tested with rice interactors and 76% were confirmed using wheat protein pairs. All interactions in the RAR1/SGT1/ HSP90, NPR1 and XB12 nodes were confirmed for the identified orthologous wheat proteins, whereas only forty four percent of the interactions were confirmed in the interactome node centered on XA21. We hypothesize that this reduction may be associated with a different sub-functionalization history of the multiple duplications that occurred in this gene family after the divergence of the wheat and rice lineages. The observed high conservation of interactions between proteins that serve as key regulators of the rice defense response suggests that the existing rice interactome can be used to predict interactions in wheat. Such predictions are less reliable for nodes that have undergone a different history of duplications and sub-functionalization in the two lineages.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
56.9
作者：
Austin, MJ;Muskett, P;Parker, JE
通讯作者：
Parker, JE
影响因子：
11.4
作者：
Hubert, DA;Tornero, P;Dangl, JL
通讯作者：
Dangl, JL
影响因子：
7.2
作者：
Halterman, DA;Wise, RP
通讯作者：
Wise, RP
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN