Overexpression of SGLT2 in the kidney of a P. gingivalis LPS-induced diabetic nephropathy mouse model.
Overexpression of SGLT2 in the kidney of a P. gingivalis LPS-induced diabetic nephropathy mouse model.
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DOI：
10.1186/s12882-021-02506-8
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发表时间：
2021-08-23
期刊：
影响因子：
2.3
通讯作者：
Sawa Y
中科院分区：
文献类型：
--
作者：
Kajiwara K;Sawa Y
据报道，糖尿病肾脏中钠 - 葡萄糖协同转运蛋白2（SGLT2）过度表达。也已确定糖尿病肾小球内皮表达Toll样受体TLR2和TLR4。本研究旨在检测Toll样受体2/4配体牙龈卟啉单胞菌（P.）脂多糖（Pg - LPS）在小鼠糖尿病肾病中对肾脏SGLT2的诱导作用。
对链脲佐菌素（STZ）诱导的糖尿病ICR小鼠（STZ - ICR）、给予Pg - LPS的健康ICR小鼠（LPS - ICR）以及Pg - LPS诱导肾病的糖尿病ICR小鼠肾脏（LPS - STZ）进行免疫组织化学研究和组织RT - PCR分析。
在血糖水平的定量分析中，LPS - STZ组达到600mg/dl的平均时间比STZ - ICR组更短。LPS - STZ组血糖水平的升高比STZ - ICR组明显更陡。根据这些数据，LPS - STZ模型显示出明显的葡萄糖不耐受。SGLT2在LPS - STZ组整个肾实质中的表达比LPS - ICR组或STZ - ICR组明显更强。在LPS - STZ组肾脏的肾小管内和肾小管周围以及肾小球中均观察到SGLT2的表达。通过组织实时PCR和细胞ELISA分析，SGLT2基因和蛋白质在LPS - STZ组中的表达比LPS - ICR组或STZ - ICR组明显更强。LPS - ICR组和STZ - ICR组肾脏中肾脏SGLT2的产生没有差异。
在伴有牙龈卟啉单胞菌LPS的糖尿病肾脏中，SGLT2出现异常高的肾脏表达。牙周炎可能是糖尿病肾病以及糖尿病的一个加重因素。
The overexpression of sodium-glucose cotransporter 2 (SGLT2) in diabetic kidneys has been reported. It has also been established that the diabetic glomerular endothelium expresses the toll-like receptors TLR2 and TLR4. The present study aims to examine the renal SGLT2 induction by the TLR2/4 ligand Porphyromonas (P.) gingivalis lipopolysaccharide (Pg-LPS) in mouse diabetic nephropathy. Immunohistochemical study and tissue RT-PCR analyses were performed on mouse kidneys in streptozotocin (STZ)-induced diabetic ICR mice (STZ-ICR), in healthy ICR mice administered Pg-LPS (LPS-ICR), and in diabetic ICR mouse kidneys with Pg-LPS-induced nephropathy (LPS-STZ). In the quantitative analysis of blood sugar levels, the mean time to reach 600 mg/dl was shorter in the LPS-STZ than in the STZ-ICR kidneys. The rise in blood glucose levels was significantly steeper in the LPS-STZ than in the STZ-ICR kidneys. According to these data the LPS-STZ model suggests a marked glucose intolerance. The expression of SGLT2 was significantly stronger in the whole of the renal parenchyma of the LPS-STZ than in the LPS-ICR or in the STZ-ICR. The expression of SGLT2 was observed both in the renal tubules and around the renal tubules, and in the glomeruli of the LPS-STZ kidneys. In the analysis by tissue real-time PCR and cell ELISA, the expression of the SGLT2 gene and protein was significantly stronger in the LPS-STZ than in the LPS-ICR or in the STZ-ICR. There were no differences in the renal SGLT2 production in the LPS-ICR and the STZ-ICR kidneys. Abnormally high renal expression of SGLT2 occurs in diabetic kidneys with P. gingivalis LPS. Periodontitis may be an exacerbating factor in diabetic nephropathy as well as in diabetes.
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DOI：
10.1016/s1532-3382(12)70006-4
发表时间：
2012-09-01
期刊：
The journal of evidence-based dental practice
影响因子：
--
作者：
Otomo-Corgel, Joan;Pucher, Jeffery J;Reynolds, Mark A
通讯作者：
Reynolds, Mark A
影响因子：
4.2
作者：
Li, Fanglin;Yang, Niansheng;Yu, Xueqing
通讯作者：
Yu, Xueqing
影响因子：
8.2
作者：
Ghezzi C;Loo DDF;Wright EM
通讯作者：
Wright EM
DOI：
10.1152/ajprenal.00319.2012
发表时间：
2012-10-01
影响因子：
4.2
作者：
Kaur, Harmeet;Chien, Alexander;Jialal, Ishwarlal
通讯作者：
Jialal, Ishwarlal
影响因子：
18.6
作者：
Garcia, RI;Henshaw, MM;Krall, EA
通讯作者：
Krall, EA