Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa.
Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa.
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DOI：
10.1186/1471-2407-5-41
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发表时间：
2005-04-18
期刊：
影响因子：
3.8
通讯作者：
Parkkila, S
中科院分区：
文献类型：
--
作者：
Kummola, L;Hämäläinen, JM;Kivelä, J;Kivelä, AJ;Saarnio, J;Karttunen, T;Parkkila, S
碳酸酐酶（CA）同工酶可能在癌症发展中起重要作用。一些同工酶控制肿瘤中的pH稳态，似乎可以调节癌细胞的行为。CA XIII是CA基因家族的最新成员。它是一种在许多正常组织中表达的胞质同工酶。本研究旨在研究CA XIII表达在前瞻性收集的结直肠肿瘤样本。肿瘤和正常组织标本均来自同一患者。使用CA XIII特异性抗体和免疫组织化学染色方法进行分析。为了比较，对组织切片进行其他胞质同工酶CA I和II的免疫染色。结果表明，与正常组织相比，CA XIII在肿瘤细胞中的表达下调。在癌样品中检测到最低信号。CA I和II的表达模式相当平行。结肠直肠癌中胞质CA I、II和XIII的下调可能是由于8号染色体上共同转录因子水平降低或紧密连锁的CA 1、CA 2和CA 13等位基因丢失所致。它们作为肿瘤抑制剂的可能作用应进一步评估。
Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples. Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II. The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II. The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated.
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影响因子：
6.4
作者：
Aragane, H;Sakakura, C;Yamagishi, H
通讯作者：
Yamagishi, H
影响因子：
4
作者：
Montcourrier, P;Silver, I;Rochefort, H
通讯作者：
Rochefort, H
影响因子：
4.8
作者：
Mori, K;Ogawa, Y;Nakao, K
通讯作者：
Nakao, K
影响因子：
29.4
作者：
MORI, M;STANIUNAS, RJ;CHEN, LB
通讯作者：
CHEN, LB
DOI：
10.1073/pnas.97.4.1677
发表时间：
2000-02-15
影响因子：
11.1
作者：
Shah, GN;Hewett-Emmett, D;Sly, WS
通讯作者：
Sly, WS