Spatially heterogeneous epidermal growth factor release from microporous annealed particle (MAP) hydrogel for improved wound closure.
Spatially heterogeneous epidermal growth factor release from microporous annealed particle (MAP) hydrogel for improved wound closure.
复制标题
微孔退火颗粒 (MAP) 水凝胶释放空间异质表皮生长因子，以改善伤口闭合。
DOI：
10.1039/d1tb00715g
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发表时间：
2021-09-15
期刊：
影响因子：
--
通讯作者：
Griffin D
中科院分区：
文献类型：
--
作者：
Pruett L;Ellis R;McDermott M;Roosa C;Griffin D
微孔退火颗粒（MAP）水凝胶是一种很有前途的支架平台技术，可促进损伤组织环境中的立即组织整合。生长因子的添加具有加速组织整合和增强支架介导的愈合的潜力。生长因子释放支架面临有限的溶解蛋白质货架稳定性的翻译障碍;然而，为了解决这个障碍，我们提出了一种冻干的MAP支架，其可以在使用前直接有效地再水化。我们的新方法包括一种异质MAP支架，其中5%的微凝胶含有1μg/mL的载有表皮生长因子（EGF）的固定化肝素。我们证明，这些支架，这是直接加载EGF冻干后保持相同的性能被动加载通过扩散到水溶胀的微凝胶，包括EGF释放曲线和细胞迁移的研究，没有显着差异的支架。此外，这些异质支架表现出体外细胞迁移的显著增加和体内更快的再上皮化。MAP支架在空间异质性释放生长因子方面的研究进展对改善复杂伤口的治疗和促进生长因子释放支架领域的发展具有巨大的潜力。我们提出了冻干肝素微凝胶，它可以直接与表皮生长因子再水化，并与PEG微凝胶混合，形成异质MAP支架。这些释放EGF的MAP支架促进加速皮肤伤口愈合。
Microporous Annealed Particle (MAP) hydrogel has been a promising scaffold platform technology to promote immediate tissue integration in injured tissue environments. The addition of growth factors has the potential to accelerate tissue integration and enhance scaffold-mediated healing. Growth factor releasing scaffolds face the translational hurdle of limited solubilized protein shelf stability; however, to address this hurdle we present a lyophilized MAP scaffold which can be effectively rehydrated directly prior to use. Our new approach includes a heterogenous MAP scaffold wherein 5% of the microgels contain immobilized heparin loaded with epidermal growth factor (EGF) at 1μg/mL. We demonstrate that these scaffolds, which are directly loaded with EGF following lyophilization maintain equivalent properties to scaffolds loaded passively via diffusion into water-swollen microgels, including EGF release profiles and cell migration studies that did not significantly differ. Further, these heterogeneous scaffolds exhibit a significant increase in cellular migration in vitro and quicker re-epithelialization in vivo. This progress on spatially heterogenous growth factor release from MAP scaffolds has great potential to improve complex wound treatment and advance the field of growth factor releasing scaffolds. We present lyophilized heparin microgels which can be directly rehydrated with Epidermal Growth Factor and mixed with PEG microgels to form heterogenous MAP scaffolds. These EGF-Releasing MAP scaffolds promote accelerated dermal wound healing.
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