Bioactive effects of silica nanoparticles on bone cells are size, surface, and composition dependent.
Bioactive effects of silica nanoparticles on bone cells are size, surface, and composition dependent.
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DOI：
10.1016/j.actbio.2018.10.018
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发表时间：
2018-12
影响因子：
9.7
通讯作者：
Beck, George R., Jr.
中科院分区：
文献类型：
--
作者：
Ha, Shin-Woo;Viggeswarapu, Manjula;Habib, Mark M.;Beck, George R., Jr.
已经证明基于二氧化硅的纳米颗粒具有针对骨骼的内在生物活性，并且通过促进骨形成成骨细胞的分化同时抑制骨再吸收破骨细胞的分化来发挥作用。围绕纳米医学的兴奋部分围绕着改变物理化学性质（包括大小，成分和表面电荷）的几乎无限的可能性。到目前为止，很少有研究试图操纵这些特征，以优化复杂的生物学结果。为此，合成了各种尺寸（50-450 nm）、不同表面性质（OH、CO2 H、NR 4+、mNH 2）和不同组成（二氧化硅、金和聚苯乙烯）的球形二氧化硅纳米颗粒，并评估其对骨骼细胞的生物活性。成骨细胞活性的组成和大小变量的影响最大，而破骨细胞的表面性质变化的影响最大。该研究还建立了纳米颗粒介导的Nfatc 1抑制，Nfatc 1是破骨细胞分化的关键转录调节因子，确定了一种新的作用机制。总的来说，该研究强调了在生物活性纳米颗粒的设计过程中，不仅要考虑可以操纵的无数物理特性，而且要考虑靶细胞的特性在决定生物学结果方面同样起着不可或缺的作用。
Silica based nanoparticles have been demonstrated to have intrinsic biologic activity towards the skeleton and to function by promoting the differentiation of bone forming osteoblasts while inhibiting the differentiation of bone resorbing osteoclasts. The excitement surrounding nanomedicine in part revolves around the almost unlimited possibilities for varying the physicochemical properties including size, composition, and surface charge. To date few studies have attempted to manipulate these characteristics in concert to optimize a complex biologic outcome. Towards this end, spherical silica nanoparticles of various sizes (50–450 nm), of different surface properties (OH, CO2H, NR4+, mNH2), and of different composition (silica, gold, and polystyrene) were synthesized and evaluated for biological activity toward skeletal cells. Osteoblast activity was most influenced by composition and size variables, whereas osteoclasts were most affected by surface property variation. The study also establishes nanoparticle mediated suppression of Nfatc1, a key transcriptional regulator for osteoclast differentiation, identifying a novel mechanism of action. Collectively, the study highlights how during the design of bioactive nanoparticles, it is vital to consider not only the myriad of physical properties that can be manipulated, but also that the characteristics of the target cell plays an equally integral role in determining biological outcome.
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DOI：
10.1039/b902195g
发表时间：
2009-05-28
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Ha SW;Camalier CE;Beck GR Jr;Lee JK
通讯作者：
Lee JK
影响因子：
5.6
作者：
Camalier, Corinne E.;Yi, Ming;Yu, Li-Rong;Hood, Brian L.;Conrads, Kelly A.;Lee, Young Jae;Lin, Yiming;Garneys, Laura M.;Bouloux, Gary F.;Young, Matthew R.;Veenstra, Timothy D.;Stephens, Robert M.;Colburn, Nancy H.;Conrads, Thomas P.;Beck, George R., Jr.
通讯作者：
Beck, George R., Jr.
影响因子：
8
作者：
Fröhlich E
通讯作者：
Fröhlich E
影响因子：
1.2
作者：
Ha, Shin-Woo;Camalier, Corinne E.;Lee, Jin-Kyu
通讯作者：
Lee, Jin-Kyu
影响因子：
3.4
作者：
Bharti, Bhuvnesh;Meissner, Jens;Findenegg, Gerhard H.
通讯作者：
Findenegg, Gerhard H.