Neuronal porosome lipidome.
Neuronal porosome lipidome.
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DOI：
10.1111/jcmm.12383
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发表时间：
2014-10
影响因子：
5.3
通讯作者：
Jena BP
中科院分区：
文献类型：
--
作者：
Lewis KT;Maddipati KR;Taatjes DJ;Jena BP
杯状脂蛋白结构称为多孔体是细胞质膜上的通用分泌门户，分泌囊泡在此处短暂停靠并融合以释放囊内内容物。在神经元中，多孔体测量为15 nm，由近40种蛋白质组成，其中包括SNARE、离子通道、Gαo G蛋白和几种结构蛋白。早期的研究报道了特定脂质的相互作用及其对SNARE、离子通道和G蛋白功能的影响。我们自己的研究证明了胆固醇对维持神经元孔体完整性的需求，以及脂质对SNARE复合物组装的影响。为了进一步了解脂质对孔体结构和功能的影响，本研究采用质谱法测定了分离的神经元孔体的脂质组成。使用脂质结合测定，孔隙体相关的突触融合蛋白-1A的各种脂质的亲和力进行了测定。我们的质谱分析结果证明了磷脂酰肌醇磷酸（PIP）和磷脂酸（PA）在其他脂质中的存在，以及神经酰胺（Cer）、溶血磷脂酰肌醇磷酸（LPIP）和二酰基甘油（DAG）的富集存在。脂质结合试验证明了神经元多孔体与心磷脂的结合，并证实了其与PIP和PA的相关性。外源性PA改变蛋白质-蛋白质相互作用和神经递质释放的能力从该研究中得到进一步证明。
Cup-shaped lipoprotein structures called porosomes are the universal secretory portals at the cell plasma membrane, where secretory vesicles transiently dock and fuse to release intravesicular contents. In neurons, porosomes measure ∼15 nm and are comprised of nearly 40 proteins, among them SNAREs, ion channels, the Gαo G-protein and several structural proteins. Earlier studies report the interaction of specific lipids and their influence on SNAREs, ion channels and G-protein function. Our own studies demonstrate the requirement of cholesterol for the maintenance of neuronal porosome integrity, and the influence of lipids on SNARE complex assembly. In this study, to further understand the role of lipids on porosome structure-function, the lipid composition of isolated neuronal porosome was determined using mass spectrometry. Using lipid-binding assays, the affinity of porosome-associated syntaxin-1A to various lipids was determined. Our mass spectrometry results demonstrate the presence of phosphatidylinositol phosphates (PIP's) and phosphatidic acid (PA) among other lipids, and the enriched presence of ceramide (Cer), lysophosphatidylinositol phosphates (LPIP) and diacylglycerol (DAG). Lipid binding assays demonstrate the binding of neuronal porosome to cardiolipin, and confirm its association with PIP's and PA. The ability of exogenous PA to alter protein–protein interaction and neurotransmitter release is further demonstrated from the study.
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影响因子：
3.3
作者：
Lam, Alice D.;Tryoen-Toth, Petra;Stuenkel, Edward L.
通讯作者：
Stuenkel, Edward L.
DOI：
10.1016/0005-2736(78)90092-5
发表时间：
1978-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
DEPONT, JJHHM;VANPROOIJENVANEEDEN, A;BONTING, SL
通讯作者：
BONTING, SL
影响因子：
4.8
作者：
Cho, SJ;Jeftinija, K;Anderson, LL
通讯作者：
Anderson, LL
影响因子：
4.8
作者：
Haviv, Haim;Habeck, Michael;Karlish, Steven J. D.
通讯作者：
Karlish, Steven J. D.
影响因子：
56.9
作者：
Hanson, Michael A.;Roth, Christopher B.;Stevens, Raymond C.
通讯作者：
Stevens, Raymond C.