Functional changes associated with structural alterations induced by mobilization of a P element inserted in the Sex-lethal gene of Drosophila.
Functional changes associated with structural alterations induced by mobilization of a P element inserted in the Sex-lethal gene of Drosophila.
复制标题
与插入果蝇性致死基因中的 P 元件动员引起的结构改变相关的功能变化。
作者：
H. Salz;T. Cline;Paul Schedl
多功能性别决定基因内重排的遗传分析性致死允许将特定功能的变化与DNA改变相关联。通过在基因的5'侧，在坐标0处的P元件的移动来分离重排。先前的工作表明，与Sxl基因功能改变相关的重排在坐标-11和0之间的11 kb区域内发现。在这里，它表明，插入外源DNA本身，在坐标0是兼容的野生型基因功能。然而，在该点的任一侧上的序列的缺失产生突变表型。远端延伸超过坐标-6.5kb的缺失导致无效表型，而较小的远端缺失或近端缺失仅消除一些Sxl功能。
Genetic analysis of rearrangements within the multifunctional sex determining gene Sex-lethal has allowed correlation of changes in specific functions with DNA alterations. Rearrangements were isolated by mobilization of a P element which is on the 5' side of the gene, at coordinate 0. Previous work has shown that rearrangements associated with alterations in Sxl gene function are found within an 11-kb region between coordinates-11 and 0. Here it is shown that insertion of foreign DNA, per se, at coordinate 0 is compatible with wild-type gene function. However, deletion of sequences on either side of this point generates a mutant phenotype. Deletions extending distally beyond coordinate -6.5 kb result in a null phenotype, whereas smaller distal deletions or proximal deletions eliminate only some Sxl functions.
果蝇中 X 连锁雌性不育位点。
影响因子：
3.3
作者：
Perrimon,N;Mohler,D;Engstrom,L;Mahowald,AP
通讯作者：
Mahowald,AP